imaging of repetitive DNA sequences via
GFP-tagged polydactyl zinc finger proteins.
Nucleic Acids Res 35:e107
9. Sanjana NE, Cong L, Zhou Y, Cunniff MM,
Feng G, Zhang F (2012) A transcription
activator-like effector toolbox for genome
engineering. Nat Protoc 7:171–192
10. Gaj T, Gersbach CA, Barbas CF (2013) ZFN,
TALEN, and CRISPR/Cas-based methods for
genome engineering. Trends Biotechnol
31:397–405
11. Miyanari Y, Ziegler-Birling C, Torres-Padilla
M-E (2013) Live visualization of chromatin
dynamics with fluorescent TALEs. Nat Struct
Mol Biol 20:1321–1324
12. Ma H, Reyes-Gutierrez P, Pederson T (2013)
Visualization of repetitive DNA sequences in
human chromosomes with transcription
activator-like effectors. Proc Natl Acad Sci U
S A 110:21048–21053
13. Fujimoto S, Sugano SS, Kuwata K, Osakabe K,
Matsunaga S (2016) Visualization of specific
repetitive genomic sequences with fluorescent
TALEs in Arabidopsis thaliana. J Exp Bot
67:6101–6110.
https://doi.org/10.1093/
jxb/erw371
14. Chen B, Gilbert LA, Cimini BA,
Schnitzbauer J, Zhang W, Li GW, Park J,
Blackburn EH, Weissman JS, Qi LS, Huang B
(2013) Dynamic imaging of genomic loci in
living human cells by an optimized CRISPR/
Cas system. Cell 155:1479–1491. https://doi.
org/10.1016/j.cell.2013.12.001
15. Chen H, Choi J, Bailey S (2014) Cut site selection by the two nuclease domains of the Cas9
RNA-guided endonuclease. J Biol Chem
289:13284–13294.
https://doi.org/10.
1074/jbc.M113.539726
16. Qi LS, Larson MH, Gilbert LA, Doudna JA,
Weissman JS, Arkin AP, Lim WA (2013)
Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene
expression. Cell 152:1173–1183
17. Lane AB, Strzelecka M, Ettinger A, Grenfell
AW, Wittmann T, Heald R (2015) Enzymatically generated CRISPR libraries for genome
labeling and screening. Dev Cell 34:373–378.
https://doi.org/10.1016/j.devcel.2015.06.
003
18. Anton T, Bultmann S, Leonhardt H, Markaki Y
(2014) Visualization of specific DNA
sequences in living mouse embryonic stem
cells with a programmable fluorescent
CRISPR/Cas system. Nucleus 5:163–172.
https://doi.org/10.4161/nucl.2848
19. Xue Y, Murat A (2018) Live-cell imaging of
chromatin condensation dynamics by CRISPR.
iScience 4:216–235. https://doi.org/10.
1016/j.isci.2018.06.001
20. Dreissig S, Schiml S, Schindele P, Weiss O,
Rutten T, Schubert V, Gladilin E, Mette MF,
Puchta H, Houben A (2017) Live-cell CRISPR
imaging in plants reveals dynamic telomere
movements. Plant J 91:565–573
21. Fujimoto S, Matsunaga S (2017) Visualization
of chromatin loci with transiently expressed
CRISPR/Cas9
in
plants.
Cytologia
82:559–562
22. Fauser F, Schiml S, Puchta H (2014) Both
CRISPR/Cas-based nucleases and nickases
can be used efficiently for genome engineering
in Arabidopsis thaliana. Plant J 79:348–359
23. Steinert J, Schiml S, Fauser F, Puchta H (2015)
Highly efficient heritable plant genome engineering using Cas9 orthologues from Streptococcus thermophilus and Staphylococcus aureus.
Plant J 84:1295–1305
356
Solmaz Khosravi et al.
GFP-tagged polydactyl zinc finger proteins.
Nucleic Acids Res 35:e107
9. Sanjana NE, Cong L, Zhou Y, Cunniff MM,
Feng G, Zhang F (2012) A transcription
activator-like effector toolbox for genome
engineering. Nat Protoc 7:171–192
10. Gaj T, Gersbach CA, Barbas CF (2013) ZFN,
TALEN, and CRISPR/Cas-based methods for
genome engineering. Trends Biotechnol
31:397–405
11. Miyanari Y, Ziegler-Birling C, Torres-Padilla
M-E (2013) Live visualization of chromatin
dynamics with fluorescent TALEs. Nat Struct
Mol Biol 20:1321–1324
12. Ma H, Reyes-Gutierrez P, Pederson T (2013)
Visualization of repetitive DNA sequences in
human chromosomes with transcription
activator-like effectors. Proc Natl Acad Sci U
S A 110:21048–21053
13. Fujimoto S, Sugano SS, Kuwata K, Osakabe K,
Matsunaga S (2016) Visualization of specific
repetitive genomic sequences with fluorescent
TALEs in Arabidopsis thaliana. J Exp Bot
67:6101–6110.
https://doi.org/10.1093/
jxb/erw371
14. Chen B, Gilbert LA, Cimini BA,
Schnitzbauer J, Zhang W, Li GW, Park J,
Blackburn EH, Weissman JS, Qi LS, Huang B
(2013) Dynamic imaging of genomic loci in
living human cells by an optimized CRISPR/
Cas system. Cell 155:1479–1491. https://doi.
org/10.1016/j.cell.2013.12.001
15. Chen H, Choi J, Bailey S (2014) Cut site selection by the two nuclease domains of the Cas9
RNA-guided endonuclease. J Biol Chem
289:13284–13294.
https://doi.org/10.
1074/jbc.M113.539726
16. Qi LS, Larson MH, Gilbert LA, Doudna JA,
Weissman JS, Arkin AP, Lim WA (2013)
Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene
expression. Cell 152:1173–1183
17. Lane AB, Strzelecka M, Ettinger A, Grenfell
AW, Wittmann T, Heald R (2015) Enzymatically generated CRISPR libraries for genome
labeling and screening. Dev Cell 34:373–378.
https://doi.org/10.1016/j.devcel.2015.06.
003
18. Anton T, Bultmann S, Leonhardt H, Markaki Y
(2014) Visualization of specific DNA
sequences in living mouse embryonic stem
cells with a programmable fluorescent
CRISPR/Cas system. Nucleus 5:163–172.
https://doi.org/10.4161/nucl.2848
19. Xue Y, Murat A (2018) Live-cell imaging of
chromatin condensation dynamics by CRISPR.
iScience 4:216–235. https://doi.org/10.
1016/j.isci.2018.06.001
20. Dreissig S, Schiml S, Schindele P, Weiss O,
Rutten T, Schubert V, Gladilin E, Mette MF,
Puchta H, Houben A (2017) Live-cell CRISPR
imaging in plants reveals dynamic telomere
movements. Plant J 91:565–573
21. Fujimoto S, Matsunaga S (2017) Visualization
of chromatin loci with transiently expressed
CRISPR/Cas9
in
plants.
Cytologia
82:559–562
22. Fauser F, Schiml S, Puchta H (2014) Both
CRISPR/Cas-based nucleases and nickases
can be used efficiently for genome engineering
in Arabidopsis thaliana. Plant J 79:348–359
23. Steinert J, Schiml S, Fauser F, Puchta H (2015)
Highly efficient heritable plant genome engineering using Cas9 orthologues from Streptococcus thermophilus and Staphylococcus aureus.
Plant J 84:1295–1305
356
Solmaz Khosravi et al.