the miRNA-mediated repression of protein
synthesis. RNA 10:1518–1525. https://doi.
org/10.1261/rna.7131604
14. Vasudevan S, Steitz JA (2007) AU-rich-element-mediated upregulation of translation by
FXR1 and Argonaute 2. Cell 128:1105–1118.
https://doi.org/10.1016/j.cell.2007.01.038
15. Katz ZB, Wells AL, Park HY, Wu B, Shenoy
SM, Singer RH (2012) β-Actin mRNA compartmentalization enhances focal adhesion stability and directs cell migration. Genes Dev
26:1885–1890.
https://doi.org/10.1101/
gad.190413.112
16. Buxbaum AR, Haimovich G, Singer RH
(2015) In the right place at the right time:
visualizing and understanding mRNA localization. Nat Rev Mol Cell Biol 16:95–109.
https://doi.org/10.1038/nrm3918
17. Weidenfeld I, Gossen M, Lo ¨w R, Kentner D,
Berger S, Go ¨rlich D, Bartsch D, Bujard H,
Scho ¨nig K (2009) Inducible expression of coding and inhibitory RNAs from retargetable
genomic loci. Nucleic Acids Res 37:e50–e50.
https://doi.org/10.1093/nar/gkp108
18. Halstead JM, Lionnet T, Wilbertz JH,
Wippich F, Ephrussi A, Singer RH, Chao JA
(2015) Translation. An RNA biosensor for
imaging the first round of translation from single cells to living animals. Science
347:1367–1671. https://doi.org/10.1126/
science.aaa3380
19. Lise ´ M-F, Srivastava DP, Arstikaitis P, Lett RL,
Sheta R, Viswanathan V, Penzes P, O’Connor
TP, El-Husseini A (2009) Myosin-Va-interacting protein, RILPL2, controls cell shape and
neuronal morphogenesis via Rac signaling. J
Cell Sci 122:3810–3821. https://doi.org/10.
1242/jcs.050344
20. Rosa-Ferreira C, Munro S (2011) Arl8 and
SKIP act together to link lysosomes to
kinesin-1. Dev Cell 21:1171–1178. https://
doi.org/10.1016/j.devcel.2011.10.007
21. Tinevez J-Y, Perry N, Schindelin J, Hoopes
GM, Reynolds GD, Laplantine E, Bednarek
SY, Shorte SL, Eliceiri KW (2017) TrackMate:
an open and extensible platform for singleparticle tracking. Methods 115:80–90.
https://doi.org/10.1016/j.ymeth.2016.09.
016
22. Schindelin J, Arganda-Carreras I, Frise E,
Kaynig V, Longair M, Pietzsch T, Preibisch S,
Rueden C, Saalfeld S, Schmid B, Tinevez J-Y,
White DJ, Hartenstein V, Eliceiri K,
Tomancak P, Cardona A (2012) Fiji: an opensource platform for biological-image analysis.
Nat Methods 9:676–682. https://doi.org/10.
1038/nmeth.2019
23. Monnier N, Barry Z, Park HY, Su K-C, Katz Z,
English BP, Dey A, Pan K, Cheeseman IM,
Singer RH, Bathe M (2015) Inferring transient
particle transport dynamics in live cells. Nat
Methods 12:838–840. https://doi.org/10.
1038/nmeth.3483
282
Varun Bhaskar et al.
synthesis. RNA 10:1518–1525. https://doi.
org/10.1261/rna.7131604
14. Vasudevan S, Steitz JA (2007) AU-rich-element-mediated upregulation of translation by
FXR1 and Argonaute 2. Cell 128:1105–1118.
https://doi.org/10.1016/j.cell.2007.01.038
15. Katz ZB, Wells AL, Park HY, Wu B, Shenoy
SM, Singer RH (2012) β-Actin mRNA compartmentalization enhances focal adhesion stability and directs cell migration. Genes Dev
26:1885–1890.
https://doi.org/10.1101/
gad.190413.112
16. Buxbaum AR, Haimovich G, Singer RH
(2015) In the right place at the right time:
visualizing and understanding mRNA localization. Nat Rev Mol Cell Biol 16:95–109.
https://doi.org/10.1038/nrm3918
17. Weidenfeld I, Gossen M, Lo ¨w R, Kentner D,
Berger S, Go ¨rlich D, Bartsch D, Bujard H,
Scho ¨nig K (2009) Inducible expression of coding and inhibitory RNAs from retargetable
genomic loci. Nucleic Acids Res 37:e50–e50.
https://doi.org/10.1093/nar/gkp108
18. Halstead JM, Lionnet T, Wilbertz JH,
Wippich F, Ephrussi A, Singer RH, Chao JA
(2015) Translation. An RNA biosensor for
imaging the first round of translation from single cells to living animals. Science
347:1367–1671. https://doi.org/10.1126/
science.aaa3380
19. Lise ´ M-F, Srivastava DP, Arstikaitis P, Lett RL,
Sheta R, Viswanathan V, Penzes P, O’Connor
TP, El-Husseini A (2009) Myosin-Va-interacting protein, RILPL2, controls cell shape and
neuronal morphogenesis via Rac signaling. J
Cell Sci 122:3810–3821. https://doi.org/10.
1242/jcs.050344
20. Rosa-Ferreira C, Munro S (2011) Arl8 and
SKIP act together to link lysosomes to
kinesin-1. Dev Cell 21:1171–1178. https://
doi.org/10.1016/j.devcel.2011.10.007
21. Tinevez J-Y, Perry N, Schindelin J, Hoopes
GM, Reynolds GD, Laplantine E, Bednarek
SY, Shorte SL, Eliceiri KW (2017) TrackMate:
an open and extensible platform for singleparticle tracking. Methods 115:80–90.
https://doi.org/10.1016/j.ymeth.2016.09.
016
22. Schindelin J, Arganda-Carreras I, Frise E,
Kaynig V, Longair M, Pietzsch T, Preibisch S,
Rueden C, Saalfeld S, Schmid B, Tinevez J-Y,
White DJ, Hartenstein V, Eliceiri K,
Tomancak P, Cardona A (2012) Fiji: an opensource platform for biological-image analysis.
Nat Methods 9:676–682. https://doi.org/10.
1038/nmeth.2019
23. Monnier N, Barry Z, Park HY, Su K-C, Katz Z,
English BP, Dey A, Pan K, Cheeseman IM,
Singer RH, Bathe M (2015) Inferring transient
particle transport dynamics in live cells. Nat
Methods 12:838–840. https://doi.org/10.
1038/nmeth.3483
282
Varun Bhaskar et al.