79:93–117.
https://doi.org/10.1146/
annurev-physiol-022516-034055
73. Cranfill PJ, Sell BR, Baird MA, Allen JR,
Lavagnino Z, de Gruiter HM, Kremers GJ,
Davidson MW, Ustione A, Piston DW (2016)
Quantitative assessment of fluorescent proteins. Nat Methods 13(7):557–562. https://
doi.org/10.1038/nmeth.3891
74. Scott DJ, Gunn NJ, Yong KJ, Wimmer VC,
Veldhuis NA, Challis LM, Haidar M,
Petrou S, Bathgate RAD, Griffin MDW
(2018) A novel ultra-stable, monomeric green
fluorescent protein for direct volumetric imaging of whole organs using CLARITY. Sci Rep 8
(1):667. https://doi.org/10.1038/s41598017-18045-y
75. Feng S, Sekine S, Pessino V, Li H, Leonetti
MD, Huang B (2017) Improved split fluorescent proteins for endogenous protein labeling.
Nat Commun 8(1):370. https://doi.org/10.
1038/s41467-017-00494-8
76. Slubowski CJ, Funk AD, Roesner JM, Paulissen SM, Huang LS (2015) Plasmids for
C-terminal tagging in Saccharomyces cerevisiae
that contain improved GFP proteins, Envy and
Ivy. Yeast 32(4):379–387. https://doi.org/10.
1002/yea.3065
77. Lim F, Peabody DS (1994) Mutations that
increase the affinity of a translational repressor
for
RNA.
Nucleic
Acids
Res
22
(18):3748–3752
78. Cormack BP, Valdivia RH, Falkow S (1996)
FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173:33–38
79. Zacharias DA, Violin JD, Newton AC, Tsien
RY (2002) Partitioning of lipid-modified
monomeric GFPs into membrane microdomains
of
live
cells.
Science
296
(5569):913–916. https://doi.org/10.1126/
science.1068539
80. Pedelacq JD, Cabantous S, Tran T, Terwilliger
TC, Waldo GS (2006) Engineering and characterization of a superfolder green fluorescent
protein. Nat Biotechnol 24(1):79–88. https://
doi.org/10.1038/nbt1172
81. Shaner NC, Lambert GG, Chammas A, Ni Y,
Cranfill PJ, Baird MA, Sell BR, Allen JR, Day
RN, Israelsson M, Davidson MW, Wang J
(2013) A bright monomeric green fluorescent
protein derived from Branchiostoma lanceolatum. Nat Methods 10(5):407–409. https://
doi.org/10.1038/nmeth.2413
82. Mueller F, Senecal A, Tantale K, Marie-NellyH, Ly N, Collin O, Basyuk E, Bertrand E,
Darzacq X, Zimmer C (2013) FISH-quant:
automatic counting of transcripts in 3D FISH
images. Nat Methods 10(4):277–278. https://
doi.org/10.1038/nmeth.2406
83. Carpenter AE, Jones TR, Lamprecht MR,
Clarke C, Kang IH, Friman O, Guertin DA,
Chang JH, Lindquist RA, Moffat J, Golland P,
Sabatini DM (2006) CellProfiler: image analysis software for identifying and quantifying cell
phenotypes. Genome Biol 7(10):R100.
https://doi.org/10.1186/gb-2006-7-10r100
144
Xavier Pichon et al.
https://doi.org/10.1146/
annurev-physiol-022516-034055
73. Cranfill PJ, Sell BR, Baird MA, Allen JR,
Lavagnino Z, de Gruiter HM, Kremers GJ,
Davidson MW, Ustione A, Piston DW (2016)
Quantitative assessment of fluorescent proteins. Nat Methods 13(7):557–562. https://
doi.org/10.1038/nmeth.3891
74. Scott DJ, Gunn NJ, Yong KJ, Wimmer VC,
Veldhuis NA, Challis LM, Haidar M,
Petrou S, Bathgate RAD, Griffin MDW
(2018) A novel ultra-stable, monomeric green
fluorescent protein for direct volumetric imaging of whole organs using CLARITY. Sci Rep 8
(1):667. https://doi.org/10.1038/s41598017-18045-y
75. Feng S, Sekine S, Pessino V, Li H, Leonetti
MD, Huang B (2017) Improved split fluorescent proteins for endogenous protein labeling.
Nat Commun 8(1):370. https://doi.org/10.
1038/s41467-017-00494-8
76. Slubowski CJ, Funk AD, Roesner JM, Paulissen SM, Huang LS (2015) Plasmids for
C-terminal tagging in Saccharomyces cerevisiae
that contain improved GFP proteins, Envy and
Ivy. Yeast 32(4):379–387. https://doi.org/10.
1002/yea.3065
77. Lim F, Peabody DS (1994) Mutations that
increase the affinity of a translational repressor
for
RNA.
Nucleic
Acids
Res
22
(18):3748–3752
78. Cormack BP, Valdivia RH, Falkow S (1996)
FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173:33–38
79. Zacharias DA, Violin JD, Newton AC, Tsien
RY (2002) Partitioning of lipid-modified
monomeric GFPs into membrane microdomains
of
live
cells.
Science
296
(5569):913–916. https://doi.org/10.1126/
science.1068539
80. Pedelacq JD, Cabantous S, Tran T, Terwilliger
TC, Waldo GS (2006) Engineering and characterization of a superfolder green fluorescent
protein. Nat Biotechnol 24(1):79–88. https://
doi.org/10.1038/nbt1172
81. Shaner NC, Lambert GG, Chammas A, Ni Y,
Cranfill PJ, Baird MA, Sell BR, Allen JR, Day
RN, Israelsson M, Davidson MW, Wang J
(2013) A bright monomeric green fluorescent
protein derived from Branchiostoma lanceolatum. Nat Methods 10(5):407–409. https://
doi.org/10.1038/nmeth.2413
82. Mueller F, Senecal A, Tantale K, Marie-NellyH, Ly N, Collin O, Basyuk E, Bertrand E,
Darzacq X, Zimmer C (2013) FISH-quant:
automatic counting of transcripts in 3D FISH
images. Nat Methods 10(4):277–278. https://
doi.org/10.1038/nmeth.2406
83. Carpenter AE, Jones TR, Lamprecht MR,
Clarke C, Kang IH, Friman O, Guertin DA,
Chang JH, Lindquist RA, Moffat J, Golland P,
Sabatini DM (2006) CellProfiler: image analysis software for identifying and quantifying cell
phenotypes. Genome Biol 7(10):R100.
https://doi.org/10.1186/gb-2006-7-10r100
144
Xavier Pichon et al.