13. Chomczynski P, Sacchi N (2006) The singlestep method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction: twenty-something years on. Nat Protoc
1(2):581–585
14. Schroeder A, Mueller O, Stocker S,
Salowsky R, Leiber M, Gassmann M et al
(2006) The RIN: an RNA integrity number
for assigning integrity values to RNA measurements. BMC Mol Biol 7(1):3–16
15. Kingston RE, Chomczynski P, Sacchi N (1996)
Guanidine methods for total RNA preparation.
Curr Protoc Mol Biol 36(1):4.2.1–4.2.9
Total RNA Extraction from Leptospira
51