48:2037–2042.
https://doi.org/10.1128/
JCM.02541-09
3. Nervig RM, Sebring RW, Scheevel KF (1986)
The enumeration of Leptospira interrogans serovar pomona by a bioluminescence ATP assay.
J Biol Stand 14:21–24
4. Casanovas-Massana A, Pedra GG, Wunder EA
Jr, Diggle PJ, Begon M, Ko AI (2018) Quantification of Leptospira interrogans survival in soil
and water microcosms. Appl Environ Microbiol 84(13). https://doi.org/10.1128/AEM.
00507-18
5. Humberd CM, Murray CK, Stuart SK, Reeb
BA, Hospenthal DR (2005) Enumerating leptospires using the coulter counter. Am J Trop
Med Hyg 73:962–963
6. Steen HB (2004) Flow cytometer for measurement of the light scattering of viral and other
submicroscopic particles. Cytometry A
57A:94–99. https://doi.org/10.1002/cyto.a.
10115
7. Bakker RG, Li C, Miller MR, Cunningham C,
Charon NW (2007) Identification of specific
chemoattractants and genetic complementation of a Borrelia burgdorferi chemotaxis
mutant: flow cytometry-based capillary tube
chemotaxis assay. Appl Environ Microbiol
73:1180–1188.
https://doi.org/10.1128/
AEM.01913-06
8. Orth R, O’Brien-Simpson N, Dashper S,
Walsh K, Reynolds E (2010) An efficient
method for enumerating oral spirochetes
using flow cytometry. J Microbiol Methods
80:123–128.
https://doi.org/10.1016/j.
mimet.2009.11.006
9. Fontana C, Crussard S, Simon-Dufay N,
Pialot D, Bomchil N, Reyes J (2017) Use of
flow cytometry for rapid and accurate enumeration of live pathogenic Leptospira strains. J
Microbiol Methods 132:34–40. https://doi.
org/10.1016/j.mimet.2016.10.013
10. Gatza E, Pen ˜a PV, Srienc F, Overton T, Lavarreda CA, Rogers CE (2012) Bioprocess monitoring with the BD Accuri™ C6 flow
cytometer. BD Biosciences White Paper
40
Ce ´ lia Fontana and Jean Reyes
https://doi.org/10.1128/
JCM.02541-09
3. Nervig RM, Sebring RW, Scheevel KF (1986)
The enumeration of Leptospira interrogans serovar pomona by a bioluminescence ATP assay.
J Biol Stand 14:21–24
4. Casanovas-Massana A, Pedra GG, Wunder EA
Jr, Diggle PJ, Begon M, Ko AI (2018) Quantification of Leptospira interrogans survival in soil
and water microcosms. Appl Environ Microbiol 84(13). https://doi.org/10.1128/AEM.
00507-18
5. Humberd CM, Murray CK, Stuart SK, Reeb
BA, Hospenthal DR (2005) Enumerating leptospires using the coulter counter. Am J Trop
Med Hyg 73:962–963
6. Steen HB (2004) Flow cytometer for measurement of the light scattering of viral and other
submicroscopic particles. Cytometry A
57A:94–99. https://doi.org/10.1002/cyto.a.
10115
7. Bakker RG, Li C, Miller MR, Cunningham C,
Charon NW (2007) Identification of specific
chemoattractants and genetic complementation of a Borrelia burgdorferi chemotaxis
mutant: flow cytometry-based capillary tube
chemotaxis assay. Appl Environ Microbiol
73:1180–1188.
https://doi.org/10.1128/
AEM.01913-06
8. Orth R, O’Brien-Simpson N, Dashper S,
Walsh K, Reynolds E (2010) An efficient
method for enumerating oral spirochetes
using flow cytometry. J Microbiol Methods
80:123–128.
https://doi.org/10.1016/j.
mimet.2009.11.006
9. Fontana C, Crussard S, Simon-Dufay N,
Pialot D, Bomchil N, Reyes J (2017) Use of
flow cytometry for rapid and accurate enumeration of live pathogenic Leptospira strains. J
Microbiol Methods 132:34–40. https://doi.
org/10.1016/j.mimet.2016.10.013
10. Gatza E, Pen ˜a PV, Srienc F, Overton T, Lavarreda CA, Rogers CE (2012) Bioprocess monitoring with the BD Accuri™ C6 flow
cytometer. BD Biosciences White Paper
40
Ce ´ lia Fontana and Jean Reyes