Fig. 3 Enhancement of Leptospira detection by staining bacteria and thresholding on fluorescence. FCM
morphology (FSC/SSC) and fluorescence (FL1/FL3) profiles of (a) unstained bacteria with a threshold at 1000
on FSC, (b) stained bacteria with a threshold at 1000 on FSC, and (c) stained bacteria with a threshold at 1000
on FL1. The red lines represent the thresholds
Fig. 4 Discrimination of live and dead bacteria based on fluorescence profiles. FCM fluorescence (FL1/FL3)
profiles of a stained (a) fresh viable Leptospira culture, (b) heat-killed Leptospira culture, and (c) mix of 50%
fresh and 50% heat-killed Leptospira cultures. The drawn green “Live” gate contains viable bacteria whereas
the red “Dead” gate contains dying and dead ones. Percentages represent population proportion in each gate
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Ce ´ lia Fontana and Jean Reyes
morphology (FSC/SSC) and fluorescence (FL1/FL3) profiles of (a) unstained bacteria with a threshold at 1000
on FSC, (b) stained bacteria with a threshold at 1000 on FSC, and (c) stained bacteria with a threshold at 1000
on FL1. The red lines represent the thresholds
Fig. 4 Discrimination of live and dead bacteria based on fluorescence profiles. FCM fluorescence (FL1/FL3)
profiles of a stained (a) fresh viable Leptospira culture, (b) heat-killed Leptospira culture, and (c) mix of 50%
fresh and 50% heat-killed Leptospira cultures. The drawn green “Live” gate contains viable bacteria whereas
the red “Dead” gate contains dying and dead ones. Percentages represent population proportion in each gate
36
Ce ´ lia Fontana and Jean Reyes