5. Trim away excess tubing from the tied end using sterile scissors
(Fig. 2d, e). The resulting filled DMC should be ~1.5–2 in.
(Fig. 2f). Larger DMCs may interfere with normal intestinal or
bladder functions.
6. Place filled DMC into a clean, sterile 50-ml conical tube.
7. Repeat steps 2–6 until all bags are filled. Proceed as soon as
possible to the surgical procedure (see Note 10).
3.3.2 Peritoneal
Implantation Procedure
1. Turn on glass bead sterilizer and circulating water heating pad
to warm up beforehand.
2. Open one pack of chlorhexidine/isopropanol swab stick (see
Note 4), sterile surgical tools, scalpel blade, applicator and
clips, suture, and surgical sterile gloves.
3. Anesthetize animal by intramuscular injection with the ketamine/xylazine/acepromazine cocktail.
4. Apply a small amount of ophthalmic ointment to each eye.
Using forceps, gently place the animal’s tongue to either side
of the mouth; this is done to ensure that the animal’s airway is
not obstructed by the tongue or bedding material.
5. Administer preoperative analgesia (e.g., carprofen, subcutaneously) (see Note 3).
6. Gently shave the abdomen using clippers (see Note 11).
7. Remove away any loose trimmed fur using alcohol prep pads
(see Note 12).
Fig. 2 Filling and preparation of dialysis membrane chambers. A dialysis tubing previously tied at one end and
sterilized is filled with Leptospira culture using a 10-ml serological pipette (a); the air remaining inside the
tubing is removed, the end is twisted (b) and tied using a simple overhand knot (c), and the remaining tubing
(d) is trimmed away using sterile scissors (e). The DMC bag should be taut and around 1.5–2 in long (f)
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Andre Alex Grassmann and Melissa J. Caimano
(Fig. 2d, e). The resulting filled DMC should be ~1.5–2 in.
(Fig. 2f). Larger DMCs may interfere with normal intestinal or
bladder functions.
6. Place filled DMC into a clean, sterile 50-ml conical tube.
7. Repeat steps 2–6 until all bags are filled. Proceed as soon as
possible to the surgical procedure (see Note 10).
3.3.2 Peritoneal
Implantation Procedure
1. Turn on glass bead sterilizer and circulating water heating pad
to warm up beforehand.
2. Open one pack of chlorhexidine/isopropanol swab stick (see
Note 4), sterile surgical tools, scalpel blade, applicator and
clips, suture, and surgical sterile gloves.
3. Anesthetize animal by intramuscular injection with the ketamine/xylazine/acepromazine cocktail.
4. Apply a small amount of ophthalmic ointment to each eye.
Using forceps, gently place the animal’s tongue to either side
of the mouth; this is done to ensure that the animal’s airway is
not obstructed by the tongue or bedding material.
5. Administer preoperative analgesia (e.g., carprofen, subcutaneously) (see Note 3).
6. Gently shave the abdomen using clippers (see Note 11).
7. Remove away any loose trimmed fur using alcohol prep pads
(see Note 12).
Fig. 2 Filling and preparation of dialysis membrane chambers. A dialysis tubing previously tied at one end and
sterilized is filled with Leptospira culture using a 10-ml serological pipette (a); the air remaining inside the
tubing is removed, the end is twisted (b) and tied using a simple overhand knot (c), and the remaining tubing
(d) is trimmed away using sterile scissors (e). The DMC bag should be taut and around 1.5–2 in long (f)
236
Andre Alex Grassmann and Melissa J. Caimano