3. Adjust the color scale in Image Adjust menu from a minimum
of 1.0 Â 10
4 to 1.0 Â 10
6 ; this allows background signal to be
removed for presentations without changing the detected signal value, saved for quantification analysis.
4. When this ROI is drawn, copy and paste ROI, selected by
right-clicking on ROI (maintaining the same ROI size), to
the other individuals imaged in the same picture (Fig. 5).
5. If needed, reposition the ROI to the selected region but without modifying the initial ROI size.
Fig. 5 ROI analysis in infected mice. To quantify the level of light emitted in each mouse, the region of interest
(ROI, shown in red) is defined and applied to all mice within the same experimental group. For each mouse,
total flux (p/s) is indicated in the respective white tag, and Avg radian (p/s/cm
2
/sr) is reported in the
measurement window
In Vivo Imaging of Bioluminescent Leptospires
157
of 1.0 Â 10
4 to 1.0 Â 10
6 ; this allows background signal to be
removed for presentations without changing the detected signal value, saved for quantification analysis.
4. When this ROI is drawn, copy and paste ROI, selected by
right-clicking on ROI (maintaining the same ROI size), to
the other individuals imaged in the same picture (Fig. 5).
5. If needed, reposition the ROI to the selected region but without modifying the initial ROI size.
Fig. 5 ROI analysis in infected mice. To quantify the level of light emitted in each mouse, the region of interest
(ROI, shown in red) is defined and applied to all mice within the same experimental group. For each mouse,
total flux (p/s) is indicated in the respective white tag, and Avg radian (p/s/cm
2
/sr) is reported in the
measurement window
In Vivo Imaging of Bioluminescent Leptospires
157