3.5 Visualization
of Periplasmic Flagella
1. Display all objects in the tomogram to visualize the periplasmic
flagella in native cellular environment using UCSF Chimera
program (Fig. 2).
2. Segment the cell body and periplasmic flagella using Segment
Map option of UCSF Chimera program (Fig. 3).
Fig. 2 Tomogram of the cell tip of Leptospira biflexa. (a–e) Slice images from tomogram of Leptospira biflexa.
Each slice is a different Z height position as indicated white dotted line in panel (f–j). Cross-section images as
indicated yellow dotted line in panel (a–e). Red arrowheads show periplasmic flagella (PF)
Fig. 3 Segmentation of cell body and periplasmic flagella. The cell body and
periplasmic flagellum are indicated in gray and pink, respectively
136
Akihiro Kawamoto
of Periplasmic Flagella
1. Display all objects in the tomogram to visualize the periplasmic
flagella in native cellular environment using UCSF Chimera
program (Fig. 2).
2. Segment the cell body and periplasmic flagella using Segment
Map option of UCSF Chimera program (Fig. 3).
Fig. 2 Tomogram of the cell tip of Leptospira biflexa. (a–e) Slice images from tomogram of Leptospira biflexa.
Each slice is a different Z height position as indicated white dotted line in panel (f–j). Cross-section images as
indicated yellow dotted line in panel (a–e). Red arrowheads show periplasmic flagella (PF)
Fig. 3 Segmentation of cell body and periplasmic flagella. The cell body and
periplasmic flagellum are indicated in gray and pink, respectively
136
Akihiro Kawamoto