Chapter 7
Endogenous Radionanomedicine:
Radiolabeling
Hongyoon Choi and Do Won Hwang
Abstract Recently, extracellular vesicles have received much attention as potential
therapeutics in a variety of fields ranging from cancer targeted delivery to regenerative medicine. In spite of promising results of therapeutic usage of extracellular
vesicles reported in various studies from small animals and in vitro investigations,
the basic physiology of administered extracellular vesicles in vivo have not yet been
fully understood. This issue limits clinical application of extracellular vesicles.
Recently developed imaging techniques of extracellular vesicles have provided
useful information including organ distribution and clearance of the injected
extracellular vesicles over time in vivo. Among several imaging techniques,
radionuclide imaging modality capable of tracing the radiolabeled extracellular
vesicles in vivo are promising in terms of clinical application, deep organ imaging
and quantitative biodistribution analyses. Here, we review the specific methods of
radiolabeling of extracellular vesicles and their initial results. We also discuss how
radiolabeling facilitates clinical translation of extracellular vesicles.
7.1 Introduction
Endogenous nanoparticles for the therapeutic delivery and diagnostic tracking are
mostly based on extracellular vesicles (EVs) [1, 2]. EVs, as naturally occurring
vesicles, convey various bioactive molecules and change the biological properties
of recipient cells [3]. And thus, their clinical application as therapeutics has
H. Choi (&) Á D. W. Hwang
Department of Nuclear Medicine, Seoul National University
College of Medicine, Seoul, Republic of Korea
e-mail: chy1000@snu.ac.kr
H. Choi Á D. W. Hwang (&)
Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate
School of Convergence Science and Technology, College of Medicine or College
of Pharmacy, Seoul National University, Seoul, Republic of Korea
e-mail: hdw6592@snu.ac.kr
© Springer International Publishing AG, part of Springer Nature 2018
D. S. Lee (ed.), Radionanomedicine, Biological and Medical Physics,
Biomedical Engineering, https://doi.org/10.1007/978-3-319-67720-0_7
141
Endogenous Radionanomedicine:
Radiolabeling
Hongyoon Choi and Do Won Hwang
Abstract Recently, extracellular vesicles have received much attention as potential
therapeutics in a variety of fields ranging from cancer targeted delivery to regenerative medicine. In spite of promising results of therapeutic usage of extracellular
vesicles reported in various studies from small animals and in vitro investigations,
the basic physiology of administered extracellular vesicles in vivo have not yet been
fully understood. This issue limits clinical application of extracellular vesicles.
Recently developed imaging techniques of extracellular vesicles have provided
useful information including organ distribution and clearance of the injected
extracellular vesicles over time in vivo. Among several imaging techniques,
radionuclide imaging modality capable of tracing the radiolabeled extracellular
vesicles in vivo are promising in terms of clinical application, deep organ imaging
and quantitative biodistribution analyses. Here, we review the specific methods of
radiolabeling of extracellular vesicles and their initial results. We also discuss how
radiolabeling facilitates clinical translation of extracellular vesicles.
7.1 Introduction
Endogenous nanoparticles for the therapeutic delivery and diagnostic tracking are
mostly based on extracellular vesicles (EVs) [1, 2]. EVs, as naturally occurring
vesicles, convey various bioactive molecules and change the biological properties
of recipient cells [3]. And thus, their clinical application as therapeutics has
H. Choi (&) Á D. W. Hwang
Department of Nuclear Medicine, Seoul National University
College of Medicine, Seoul, Republic of Korea
e-mail: chy1000@snu.ac.kr
H. Choi Á D. W. Hwang (&)
Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate
School of Convergence Science and Technology, College of Medicine or College
of Pharmacy, Seoul National University, Seoul, Republic of Korea
e-mail: hdw6592@snu.ac.kr
© Springer International Publishing AG, part of Springer Nature 2018
D. S. Lee (ed.), Radionanomedicine, Biological and Medical Physics,
Biomedical Engineering, https://doi.org/10.1007/978-3-319-67720-0_7
141