PART III ELECTRON CRYSTALLOGRAPHY OF 2D CRYSTALS
10 2D Electron Crystallography of Membrane Protein Single-,
Double-, and Multi-Layered Ordered Arrays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 227
Matthew C. Johnson, Yusuf M. Uddin, Kasahun Neselu,
and Ingeborg Schmidt-Krey
11 Sample Preparation and Data Collection for Electron Crystallographic
Studies on Membrane Protein Structures and Lipid–Protein Interaction . . . . . . . 247
Ka-Yi Chan, Chloe Du Truong, Yu-Ping Poh,
and Po-Lin Chiu
12 Single Particle Analysis for High-Resolution 2D Electron
Crystallography. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 267
Ricardo Righetto and Henning Stahlberg
PART IV MICROCRYSTAL ELECTRON DIFFRACTION (MICROED)
13 MicroED Sample Preparation and Data Collection
For Protein Crystals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 287
Guanhong Bu and Brent L. Nannenga
14 Detection of Microcrystals for CryoEM . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 299
Simon Weiss, Sandra Vergara, Guowu Lin,
and Guillermo Calero
15 Low-Dose Data Collection and Radiation Damage in MicroED . . . . . . . . . . . . . . 309
Johan Hattne
16 Automation of Continuous-Rotation Data Collection for MicroED. . . . . . . . . . . 321
M. Jason de la Cruz
17 Ab Initio Determination of Peptide Structures by MicroED. . . . . . . . . . . . . . . . . . 329
Chih-Te Zee, Ambarneil Saha, Michael R. Sawaya,
and Jose A. Rodriguez
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 349
viii
Contents