Acknowledgments
The authors would like to acknowledge the Penn State CryoElectron Microscopy Core Facility and funding provided by TSF
CURE award 4100079742.
References
1. Gan L, Jensen GJ (2012) Electron tomography
of cells. Q Rev Biophys 45:27–56
2. Beck M, Baumeister W (2016) Cryo-Electron
tomography: can it reveal the molecular sociology of cells in atomic detail? Trends Cell Biol
26:825–837
3. Ladinsky MS (2010) Micromanipulatorassisted vitreous cryosectioning and sample
preparation by high-pressure freezing. Methods Enzymol 481:165–194
4. Rigort A et al (2012) Focused ion beam micromachining of eukaryotic cells for cryoelectron
tomography.
Proc
Natl
Acad
Sci
109:4449–4454
5. Rigort A et al (2012) Automated segmentation
of electron tomograms for a quantitative
description of actin filament networks. J Struct
Biol 177:135–144
6. Lucic ´ V, Ferna ´ndez-Busnadiego R, Laugks U,
Baumeister W (2016) Hierarchical detection
and analysis of macromolecular complexes in
cryo-electron tomograms using Pyto software.
J Struct Biol 196:503–514
7. Chen M et al (2017) Convolutional neural networks for automated annotation of cellular
cryo-electron tomograms. Nat Methods
14:983–985
8. Thermo Fisher Scientific’s CryoEM University.
https://www.thermofisher.com/blog/micros
copy/cryo-em-university-an-online-electronmicroscopy-curriculum/
9. Kremer JR, Mastronarde DN, McIntosh JR
(1996) Computer visualization of threedimensional image data using IMOD. J Struct
Biol 116:71–76
10. Schrod N et al (2018) Pleomorphic linkers as
ubiquitous structural organizers of vesicles in
axons. PLoS One 13:e0197886
11. Tao C-L et al (2018) Differentiation and characterization of excitatory and inhibitory
synapses by Cryo-electron tomography and
correlative
microscopy.
J
Neurosci
38:1493–1510
12. Fischer TD, Dash PK, Liu J, Waxham MN
(2018) Morphology of mitochondria in spatially restricted axons revealed by cryo-electron
tomography. PLoS Biol 16:e2006169
48
Ryan K. Hylton et al.
The authors would like to acknowledge the Penn State CryoElectron Microscopy Core Facility and funding provided by TSF
CURE award 4100079742.
References
1. Gan L, Jensen GJ (2012) Electron tomography
of cells. Q Rev Biophys 45:27–56
2. Beck M, Baumeister W (2016) Cryo-Electron
tomography: can it reveal the molecular sociology of cells in atomic detail? Trends Cell Biol
26:825–837
3. Ladinsky MS (2010) Micromanipulatorassisted vitreous cryosectioning and sample
preparation by high-pressure freezing. Methods Enzymol 481:165–194
4. Rigort A et al (2012) Focused ion beam micromachining of eukaryotic cells for cryoelectron
tomography.
Proc
Natl
Acad
Sci
109:4449–4454
5. Rigort A et al (2012) Automated segmentation
of electron tomograms for a quantitative
description of actin filament networks. J Struct
Biol 177:135–144
6. Lucic ´ V, Ferna ´ndez-Busnadiego R, Laugks U,
Baumeister W (2016) Hierarchical detection
and analysis of macromolecular complexes in
cryo-electron tomograms using Pyto software.
J Struct Biol 196:503–514
7. Chen M et al (2017) Convolutional neural networks for automated annotation of cellular
cryo-electron tomograms. Nat Methods
14:983–985
8. Thermo Fisher Scientific’s CryoEM University.
https://www.thermofisher.com/blog/micros
copy/cryo-em-university-an-online-electronmicroscopy-curriculum/
9. Kremer JR, Mastronarde DN, McIntosh JR
(1996) Computer visualization of threedimensional image data using IMOD. J Struct
Biol 116:71–76
10. Schrod N et al (2018) Pleomorphic linkers as
ubiquitous structural organizers of vesicles in
axons. PLoS One 13:e0197886
11. Tao C-L et al (2018) Differentiation and characterization of excitatory and inhibitory
synapses by Cryo-electron tomography and
correlative
microscopy.
J
Neurosci
38:1493–1510
12. Fischer TD, Dash PK, Liu J, Waxham MN
(2018) Morphology of mitochondria in spatially restricted axons revealed by cryo-electron
tomography. PLoS Biol 16:e2006169
48
Ryan K. Hylton et al.