Microcrystal electron diffraction
(MicroED) ................................................. 84, 228,
234, 287, 288, 290, 292–294, 309–317,
321–327, 329–347
Molecular motions ........................................................ 145
N
Nanocrystals ......................................................... 321, 329
Negative stain ...................................................... 115–123,
228, 229, 231–234, 236, 243, 248, 252, 254,
255, 300, 301
Neural networks ...........................................26, 40–42, 47
Neurons .....................................................................25–48
P
Parallel illumination .....................................125–143, 259
Peel-blot .............................................................. 228, 230,
234–236, 238
Peptides ........................................................287, 329–347
Preferred orientations ......................................... 164, 165,
171, 175, 176, 179, 183, 184
Protein crystallography ................................................. 287
Protein structures.................................................... 5, 194,
198, 200, 227, 240, 241, 247–263, 268
R
Radiation damage ............................................... 4, 5, 109,
250, 268, 309–317, 330
Reconstitution ............................................................... 227
Regularized likelihood optimization............................ 145
RELION..................................................... 106, 145–159,
165, 179, 182, 275, 279, 281
S
Sample preparation ............................................... 3, 6, 50,
57, 58, 62, 64–65, 85–98, 228, 236, 249,
252–257, 270, 287–294, 311–312, 330, 332, 342
Sampling .............................................................. 151, 153,
164, 170, 271, 281, 294, 331, 343, 344
Segmentations ..........................................................14–17,
25–47, 54, 193, 195
Single particle electron cryomicroscopy ...................... 125
Single particle analysis........................................... 84, 125,
138, 163, 267–281
Structural biology .................................................. 83, 228
Structural cell biology....................................................... 3
Structure modeling ....................................................... 189
Sub-tomogram averaging ..............................14, 101, 106
Sugar embedding .......................................................... 249
T
3D classification .................................................. 145, 154,
155, 278–281
3D reconstruction................................................. 84, 126,
139, 161, 163, 257, 268
Tilt series..........................................................4, 5, 11–14,
19, 26, 31, 33, 35–39, 46, 47, 56, 62, 71, 73, 78,
88, 98–105, 108, 271
Tomography ...................................................... 25–47, 50,
56, 71, 78, 84, 85, 98, 101, 106, 138, 163, 271
2D crystallization .........................................228–234, 241
2D electron crystallography ............................... 227–243,
248, 249, 267–282
U
Uranyl acetate (UA)............................................ 118–120,
229, 230, 232, 236, 237, 242, 243, 300, 301,
303, 305, 306
Uranyl formate (UF) ..........................118–120, 242, 243
V
Validation......................................................189–219, 275
Vitrification...................................................................4, 7,
10, 11, 27, 30–32, 46, 58, 60, 61, 76, 93–95, 97,
293, 331
Y
Yeasts.............................................................57–62, 80, 90
350
CRYOEM: METHODS AND PROTOCOLS
Index