and insights into reversible or pathogenic
aggregation. Nat Struct Mol Biol 25:463–471
11. Hughes MP, Sawaya MR, Boyer DR et al
(2018) Atomic structures of low-complexity
protein segments reveal kinked β sheets that
assemble networks. Science 359:698–701
12. Hattne J, Shi D, Glynn C et al (2018) Analysis
of global and site-specific radiation damage in
cryo-EM. Structure 26:759–766.e4
13. Hattne J, Reyes FE, Nannenga BL et al (2015)
MicroED data collection and processing. Nat
Struct Mol Biol 71:353–360
14. Palatinus L, Bra ´zda P, Boullay P et al (2017)
Hydrogen positions in single nanocrystals
revealed by electron diffraction. Science
355:166–169
15. Zou X, Hovmo ¨ller S, Oleynikov P (2011) Electron crystallography: electron microscopy and
electron diffraction. Oxford University Press,
Oxford
16. Zuo JM, Spence JCH (2017) Advanced transmission electron microscopy: imaging and diffraction in nanoscience. Springer-Verlag,
New York
17. Frank J (2006) Three-dimensional electron
microscopy of macromolecular assemblies:
visualization of biological molecules in their
native state. Oxford University Press, Oxford
18. Heidler J, Pantelic R, Wennmacher JTC et al
(2019) Design guidelines for an electron diffractometer for structural chemistry and structural biology. Acta Cryst D 75:458–466
19. Passmore LA, Russo CJ (2016) Specimen
preparation for high-resolution cryo-EM.
Methods Enzymol 579:51–86
20. Knapek E, Dubochet J (1980) Beam damage
to organic material is considerably reduced in
cryo-electron microscopy. J Mol Biol
141:147–161
21. Dubochet J, Adrian M, Chang J-J et al (1988)
Cryo-electron microscopy of vitrified specimens. Q Rev Biophys 21:129–228
22. Jones C, Asay M, Kim LJ et al (2019) Characterization of reactive organometallic species via
MicroED. ACS Cent Sci 5(9):1507–1513
23. Yonekura K, Ishikawa T, Maki-Yonekura S
(2019) A new cryo-EM system for electron
3D crystallography by eEFD. J Struct Biol
206:243–253
24. Arvai A (2015) Adxv—A program to display
X-ray diffraction images
25. Kabsch W (2010) XDS. Acta Crystallogr D
Biol Crystallogr 66:125–132
26. Sheldrick GM (2008) A short history of
SHELX. Acta Cryst 64:112–122
27. Adams PD, Afonine PV, Bunko ´ czi G et al
(2010) PHENIX : a comprehensive Pythonbased system for macromolecular structure
solution. Acta Cryst 66:213–221
28. Murshudov GN, Skuba ´k P, Lebedev AA et al
(2011) REFMAC 5 for the refinement of macromolecular crystal structures. Acta Cryst
67:355–367
29. Winn MD, Ballard CC, Cowtan KD et al
(2011) Overview of the CCP4 suite and current developments. Acta Crystallogr D Biol
Crystallogr 67:235–242
30. Emsley P, Lohkamp B, Scott WG et al (2010)
Features and development of Coot. Acta Crystallogr D Biol Crystallogr 66:486–501
31. Delano W (2016) The PyMOL molecular graphics system. LLC, Schro ¨dinger
32. Grassucci RA, Taylor DJ, Frank J (2007) Preparation of macromolecular complexes for cryoelectron microscopy. Nat Protoc 2:3239–3246
33. Bra ´zda P, Palatinus L, Babor M (2019) Electron diffraction determines molecular absolute
configuration in a pharmaceutical nanocrystal.
Science 364:667–669
34. Reimer L, Kohl H (2008) Transmission Electron Microscopy: Physics of Image Formation.
Springer-Verlag, New York
35. Hattne J, Martynowycz MW, Gonen T (2019)
MicroED with the Falcon III direct electron
detector. IUCrJ 6:921–926
36. Gallagher-Jones M, Ophus C, Bustillo KC et al
(2019) Nanoscale mosaicity revealed in peptide
microcrystals by scanning electron nanodiffraction. Commun Biol 2:26
37. Tinti G, Fro ¨jdh E, van Genderen E et al (2018)
Electron crystallography with the EIGER
detector. IUCrJ 5:190–199
38. Nederlof I, van Genderen E, Li Y-W et al
(2013) A Medipix quantum area detector
allows rotation electron diffraction data collection from submicrometre three-dimensional
protein
crystals.
Biol
Crystallogr
69:1223–1230
39. Sheldrick GM (2015) SHELXT—integrated
space-group and crystal-structure determination. Acta Crystallogr A Found Adv 71:3–8
40. Peng L-M (1999) Electron atomic scattering
factors and scattering potentials of crystals.
Micron 30:625–648
41. Williams DB (1996) Transmission electron
microscopy—a textbook for materials science.
Springer, New York. https://www.springer.
com/us/book/9780387765006
348
Chih-Te Zee et al.
aggregation. Nat Struct Mol Biol 25:463–471
11. Hughes MP, Sawaya MR, Boyer DR et al
(2018) Atomic structures of low-complexity
protein segments reveal kinked β sheets that
assemble networks. Science 359:698–701
12. Hattne J, Shi D, Glynn C et al (2018) Analysis
of global and site-specific radiation damage in
cryo-EM. Structure 26:759–766.e4
13. Hattne J, Reyes FE, Nannenga BL et al (2015)
MicroED data collection and processing. Nat
Struct Mol Biol 71:353–360
14. Palatinus L, Bra ´zda P, Boullay P et al (2017)
Hydrogen positions in single nanocrystals
revealed by electron diffraction. Science
355:166–169
15. Zou X, Hovmo ¨ller S, Oleynikov P (2011) Electron crystallography: electron microscopy and
electron diffraction. Oxford University Press,
Oxford
16. Zuo JM, Spence JCH (2017) Advanced transmission electron microscopy: imaging and diffraction in nanoscience. Springer-Verlag,
New York
17. Frank J (2006) Three-dimensional electron
microscopy of macromolecular assemblies:
visualization of biological molecules in their
native state. Oxford University Press, Oxford
18. Heidler J, Pantelic R, Wennmacher JTC et al
(2019) Design guidelines for an electron diffractometer for structural chemistry and structural biology. Acta Cryst D 75:458–466
19. Passmore LA, Russo CJ (2016) Specimen
preparation for high-resolution cryo-EM.
Methods Enzymol 579:51–86
20. Knapek E, Dubochet J (1980) Beam damage
to organic material is considerably reduced in
cryo-electron microscopy. J Mol Biol
141:147–161
21. Dubochet J, Adrian M, Chang J-J et al (1988)
Cryo-electron microscopy of vitrified specimens. Q Rev Biophys 21:129–228
22. Jones C, Asay M, Kim LJ et al (2019) Characterization of reactive organometallic species via
MicroED. ACS Cent Sci 5(9):1507–1513
23. Yonekura K, Ishikawa T, Maki-Yonekura S
(2019) A new cryo-EM system for electron
3D crystallography by eEFD. J Struct Biol
206:243–253
24. Arvai A (2015) Adxv—A program to display
X-ray diffraction images
25. Kabsch W (2010) XDS. Acta Crystallogr D
Biol Crystallogr 66:125–132
26. Sheldrick GM (2008) A short history of
SHELX. Acta Cryst 64:112–122
27. Adams PD, Afonine PV, Bunko ´ czi G et al
(2010) PHENIX : a comprehensive Pythonbased system for macromolecular structure
solution. Acta Cryst 66:213–221
28. Murshudov GN, Skuba ´k P, Lebedev AA et al
(2011) REFMAC 5 for the refinement of macromolecular crystal structures. Acta Cryst
67:355–367
29. Winn MD, Ballard CC, Cowtan KD et al
(2011) Overview of the CCP4 suite and current developments. Acta Crystallogr D Biol
Crystallogr 67:235–242
30. Emsley P, Lohkamp B, Scott WG et al (2010)
Features and development of Coot. Acta Crystallogr D Biol Crystallogr 66:486–501
31. Delano W (2016) The PyMOL molecular graphics system. LLC, Schro ¨dinger
32. Grassucci RA, Taylor DJ, Frank J (2007) Preparation of macromolecular complexes for cryoelectron microscopy. Nat Protoc 2:3239–3246
33. Bra ´zda P, Palatinus L, Babor M (2019) Electron diffraction determines molecular absolute
configuration in a pharmaceutical nanocrystal.
Science 364:667–669
34. Reimer L, Kohl H (2008) Transmission Electron Microscopy: Physics of Image Formation.
Springer-Verlag, New York
35. Hattne J, Martynowycz MW, Gonen T (2019)
MicroED with the Falcon III direct electron
detector. IUCrJ 6:921–926
36. Gallagher-Jones M, Ophus C, Bustillo KC et al
(2019) Nanoscale mosaicity revealed in peptide
microcrystals by scanning electron nanodiffraction. Commun Biol 2:26
37. Tinti G, Fro ¨jdh E, van Genderen E et al (2018)
Electron crystallography with the EIGER
detector. IUCrJ 5:190–199
38. Nederlof I, van Genderen E, Li Y-W et al
(2013) A Medipix quantum area detector
allows rotation electron diffraction data collection from submicrometre three-dimensional
protein
crystals.
Biol
Crystallogr
69:1223–1230
39. Sheldrick GM (2015) SHELXT—integrated
space-group and crystal-structure determination. Acta Crystallogr A Found Adv 71:3–8
40. Peng L-M (1999) Electron atomic scattering
factors and scattering potentials of crystals.
Micron 30:625–648
41. Williams DB (1996) Transmission electron
microscopy—a textbook for materials science.
Springer, New York. https://www.springer.
com/us/book/9780387765006
348
Chih-Te Zee et al.