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105:28–34. https://doi.org/10.1016/10478477(90)90095-T
36. McMullan G, Chen S, Henderson R, Faruqi
AR (2009) Detective quantum efficiency of
electron area detectors in electron microscopy.
Ultramicroscopy 109:1126–1143. https://
doi.org/10.1016/j.ultramic.2009.04.002
37. Milazzo A-C, Cheng A, Moeller A et al (2011)
Initial evaluation of a direct detection device
detector for single particle cryo-electron
microscopy. J Struct Biol 176:404–408.
https://doi.org/10.1016/j.jsb.2011.09.002
38. Gilbert P (1972) Iterative methods for the
three-dimensional reconstruction of an object
from projections. J Theor Biol 36:105–117.
https://doi.org/10.1016/0022-5193(72)
90180-4
39. Radermacher M (2006) In: Frank J
(ed) Weighted Back-projection methods
BT-electron tomography: methods for threedimensional visualization of structures in the
cell. Springer, New York, NY, pp 245–273
40. Wan W, Briggs JAG (2016) Chapter thirteencryo-electron tomography and subtomogram
averaging. In: Crowther RA (ed) Methods
enzymol. Academic Press, New York, pp
329–367
41. Mahamid J, Pfeffer S, Schaffer M et al (2016)
Visualizing the molecular sociology at the
HeLa cell nuclear periphery. Science
351:969–972. https://doi.org/10.1126/sci
ence.aad8857
42. Mu ¨hleip AW, Dewar CE, Schnaufer A et al
(2017) In situ structure of trypanosomal ATP
synthase dimer reveals a unique arrangement of
catalytic subunits. Proc Natl Acad Sci U S A
114:992–997.
https://doi.org/10.1073/
pnas.1612386114
43. Schur FKM, Hagen WJH, de Marco A, Briggs
JAG (2013) Determination of protein structure at 8.5A ˚ resolution using cryo-electron
tomography and sub-tomogram averaging. J
Struct Biol 184:394–400. https://doi.org/
10.1016/j.jsb.2013.10.015
44. Mastronarde DN (2005) Automated electron
microscope tomography using robust prediction of specimen movements. J Struct Biol
152:36–51. https://doi.org/10.1016/j.jsb.
2005.07.007
45. Kremer JR, Mastronarde DN, McIntosh JR
(1996) Computer visualization of threedimensional image data using IMOD. J Struct
Biol 116:71–76. https://doi.org/10.1006/
jsbi.1996.0013
46. Hampton CM, Strauss JD, Ke Z et al (2017)
Correlated fluorescence microscopy and cryoelectron tomography of virus-infected or transfected mammalian cells. Nat Protoc
12:150–167.
https://doi.org/10.1038/
nprot.2016.168.
http://www.nature.com/
nprot/journal/v12/n1/abs/nprot.2016.168.
html#supplementary-information
Cryo-ET of Cellular Structures
23