Acknowledgments
I would like to thank Tamir Gonen for access to a Thermo Fisher
Talos Arctica TEM for testing and data collection, and Shian Liu
and M. Rhyan Puno for their critical reading of this manuscript.
This work is supported by the NIH/NCI Cancer Center Support
Grant P30 CA008748 and the Sloan Kettering Institute.
References
1. Tan YZ, Cheng A, Potter CS et al (2016) Automated data collection in single particle electron
microscopy. Microscopy (Oxf) 65:43–56
2. Mastronarde DN (2005) Automated electron
microscope tomography using robust prediction
of specimen movements. J Struct Biol
152:36–51
3. Yonekura K, Ishikawa T, Maki-Yonekura S
(2019) A new cryo-EM system for electron 3D
crystallography by eEFD. J Struct Biol
206:243–253
4. Suloway C, Pulokas J, Fellmann D et al (2005)
Automated molecular microscopy: the new
Leginon system. J Struct Biol 151:41–60
5. de la Cruz MJ, Martynowycz MW, Hattne J et al
(2019) MicroED data collection with SerialEM.
Ultramicroscopy 201:77–80
6. Cheng A (2013) Automation of data acquisition
in electron crystallography. In: Schmidt-Krey I,
Cheng Y (eds) Electron crystallography of soluble and membrane proteins, Methods in Molecular Biology, vol 355. Humana Press, Totowa
NJ, pp 307–312
7. Nakamura N, Shimizu Y, Shinkawa T et al
(2010) Automated specimen search in cryoTEM observation with DIFF-defocus imaging.
J Electron Microsc 59:299–310
8. Shi D, Nannenga BL, de la Cruz MJ et al (2016)
The collection of MicroED data for macromolecular crystallography. Nat Protoc 11:895–904
9. Hattne J, Reyes FE, Nannenga BL et al (2015)
MicroED data collection and processing. Acta
Crystallogr Sect A Found Adv 71:353–360
Automation of Continuous-Rotation Data Collection for MicroED
327
I would like to thank Tamir Gonen for access to a Thermo Fisher
Talos Arctica TEM for testing and data collection, and Shian Liu
and M. Rhyan Puno for their critical reading of this manuscript.
This work is supported by the NIH/NCI Cancer Center Support
Grant P30 CA008748 and the Sloan Kettering Institute.
References
1. Tan YZ, Cheng A, Potter CS et al (2016) Automated data collection in single particle electron
microscopy. Microscopy (Oxf) 65:43–56
2. Mastronarde DN (2005) Automated electron
microscope tomography using robust prediction
of specimen movements. J Struct Biol
152:36–51
3. Yonekura K, Ishikawa T, Maki-Yonekura S
(2019) A new cryo-EM system for electron 3D
crystallography by eEFD. J Struct Biol
206:243–253
4. Suloway C, Pulokas J, Fellmann D et al (2005)
Automated molecular microscopy: the new
Leginon system. J Struct Biol 151:41–60
5. de la Cruz MJ, Martynowycz MW, Hattne J et al
(2019) MicroED data collection with SerialEM.
Ultramicroscopy 201:77–80
6. Cheng A (2013) Automation of data acquisition
in electron crystallography. In: Schmidt-Krey I,
Cheng Y (eds) Electron crystallography of soluble and membrane proteins, Methods in Molecular Biology, vol 355. Humana Press, Totowa
NJ, pp 307–312
7. Nakamura N, Shimizu Y, Shinkawa T et al
(2010) Automated specimen search in cryoTEM observation with DIFF-defocus imaging.
J Electron Microsc 59:299–310
8. Shi D, Nannenga BL, de la Cruz MJ et al (2016)
The collection of MicroED data for macromolecular crystallography. Nat Protoc 11:895–904
9. Hattne J, Reyes FE, Nannenga BL et al (2015)
MicroED data collection and processing. Acta
Crystallogr Sect A Found Adv 71:353–360
Automation of Continuous-Rotation Data Collection for MicroED
327