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Qi L, Chen T, Craik CS, Jan YN, Minor DL
Jr, Cheng Y, Jan LY (2017) Cryo-EM structures of the TMEM16A calcium-activated
chloride
channel.
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nature25024
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EMAN: semiautomated software for highresolution single-particle reconstructions. J
Struct Biol 128(1):82–97. https://doi.org/
10.1006/jsbi.1999.4174
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https://doi.org/10.1016/j.jsb.2012.09.006
29. Punjani A, Rubinstein JL, Fleet DJ, Brubaker
MA (2017) cryoSPARC: algorithms for rapid
unsupervised cryo-EM structure determination. Nat Methods 14(3):290–296. https://
doi.org/10.1038/nmeth.4169
30. Kucukelbir A, Sigworth FJ, Tagare HD (2014)
Quantifying the local resolution of cryo-EM
density maps. Nat Methods 11(1):63–65.
https://doi.org/10.1038/nmeth.2727
31. Vilas JL, Gomez-Blanco J, Conesa P, Melero R,
Miguel de la Rosa-Trevin J, Oton J, Cuenca J,
Marabini R, Carazo JM, Vargas J, Sorzano
COS (2018) MonoRes: automatic and accurate estimation of local resolution for electron
microscopy maps. Structure 26(2):337–344.
e334.
https://doi.org/10.1016/j.str.2017.
12.018
32. de la Rosa-Trevin JM, Oton J, Marabini R,
Zaldivar A, Vargas J, Carazo JM, Sorzano CO
(2013) Xmipp 3.0: an improved software suite
for image processing in electron microscopy. J
Struct Biol 184(2):321–328. https://doi.org/
10.1016/j.jsb.2013.09.015
33. de la Rosa-Trevin JM, Quintana A, Del
Cano L, Zaldivar A, Foche I, Gutierrez J,
Gomez-Blanco J, Burguet-Castell J, CuencaAlba J, Abrishami V, Vargas J, Oton J,
Sharov G, Vilas JL, Navas J, Conesa P,
Kazemi M, Marabini R, Sorzano CO, Carazo
JM (2016) Scipion: a software framework
toward integration, reproducibility and
validation in 3D electron microscopy. J Struct
Biol
195(1):93–99.
https://doi.org/10.
1016/j.jsb.2016.04.010
34. Chen S, McMullan G, Faruqi AR, Murshudov
GN, Short JM, Scheres SH, Henderson R
(2013) High-resolution noise substitution to
measure overfitting and validate resolution in
3D structure determination by single particle
electron cryomicroscopy. Ultramicroscopy
135:24–35. https://doi.org/10.1016/j.ultra
mic.2013.06.004
35. Dang S, Feng S, Tien J, Peters CJ, Bulkley D,
Lolicato M, Zhao J, Zuberbuhler K, Ye W,
Qi L, Chen T, Craik CS, Jan YN, Minor DL
Jr, Cheng Y, Jan LY (2017) Cryo-EM structures of the TMEM16A calcium-activated
chloride
channel.
Nature
552
(7685):426–429. https://doi.org/10.1038/
nature25024
36. Ludtke SJ, Baldwin PR, Chiu W (1999)
EMAN: semiautomated software for highresolution single-particle reconstructions. J
Struct Biol 128(1):82–97. https://doi.org/
10.1006/jsbi.1999.4174
37. Grant T, Rohou A, Grigorieff N (2018) cisTEM, user-friendly software for single-particle
image processing. elife 7:e35383. https://doi.
org/10.7554/eLife.35383
38. Rohou A (2020) Fourier shell correlation criteria for local resolution estimation. bioRxiv,
It20, 2020.03.01.972067. https://doi.org/
10.1101/2020.03.01.972067
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187