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Setting up the Talos Arctica electron microscope and Gatan K2 direct detector for highresolution cryogenic single-particle data acquisition.
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16. Janus M, Voight A (2016) EPU Software
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1–106
17. Yan R, Li K, Jiang W (2018) Defocus and
magnification dependent variation of TEM
image astigmatism. Sci Rep 8:344
18. Yan R, Li K, Jiang W (2017) Real-time detection and single-pass minimization of TEM
objective lens astigmatism. J Struct Biol
197:210–219
19. Li X, Zheng SQ, Egami K, Agard DA, Cheng Y
(2013) Influence of electron dose rate on electron counting images recorded with the K2
camera. J Struct Biol 184:251–260
144
Mark A. Herzik Jr
Membrane Proteins. Methods in Molecular
Biology (Methods and Protocols), vol 955.
Humana Press, Totowa, NJ. https://doi.org/
10.1007/978-1-62703-176-9_8
11. Herzik MA, Wu M, Lander GC (2017) Achieving better-than-3-A ˚ resolution by singleparticle cryo-EM at 200 keV. Nat Methods
14:1075–1078
12. Herzik Mark AJ, Wu M, Lander GC (2017)
Setting up the Talos Arctica electron microscope and Gatan K2 direct detector for highresolution cryogenic single-particle data acquisition.
https://doi.org/10.1038/protex.
2017.108
13. Herzik MA, Wu M, Lander GC (2019) Highresolution structure determination of sub-100kDa complexes using conventional cryo-EM.
Nat Commun 10:1032
14. Suloway C et al (2005) Automated molecular
microscopy: the new Leginon system. J Struct
Biol 151:41–60
15. Mastronarde DN (2005) Automated electron
microscope tomography using robust prediction of specimen movements. J Struct Biol
152:36–51
16. Janus M, Voight A (2016) EPU Software
User’s Guide. FEI Company, Hillsboro, pp
1–106
17. Yan R, Li K, Jiang W (2018) Defocus and
magnification dependent variation of TEM
image astigmatism. Sci Rep 8:344
18. Yan R, Li K, Jiang W (2017) Real-time detection and single-pass minimization of TEM
objective lens astigmatism. J Struct Biol
197:210–219
19. Li X, Zheng SQ, Egami K, Agard DA, Cheng Y
(2013) Influence of electron dose rate on electron counting images recorded with the K2
camera. J Struct Biol 184:251–260
144
Mark A. Herzik Jr