Table 19.1
Biochemical comparison of ACC deaminase enzymes purified from different sources
Strain
K
m (mM)
k
cat (m
–
1
)
k
cat /
K
m
Optimum
pH
Optimum
temperature,
C
Structure
Reference
Pseudomonas putida
GR12-2 n.d.
n.d.
n.d.
8.5
30
Homotrimer
Jacobson et al. (1994)
Pseudomonas
sp. strain APC 1.97
70
35.5
8.5
30
Homotrimer
Honma and Shimomura (1978);
Karthikeyan et al. (2004)
Pseudomonas putida
UW4
3.4
Æ 0.2
146
Æ
5
43.9
8
22
Homotrimer
Hontzeas et al. (2004)
Methylobacterium nodulans
ORS2060
0.80
Æ 0.04 111.8
Æ 0.2
139.8 8
50
Homotetramer Fedorov et al. (2013)
Methylobacterium
radiotolerans
JCM2831
1.8
Æ 0.3
65.8
Æ
2.8
36.5
8
45
Homotetramer Fedorov et al. (2013)
Cyberlindera saturnus
2.6
n.d.
n.d.
9.0
37
Homodimer
Honma and Shimomura (1978);
Minami et al. (1998)
Penicillium citrinum
4.8
n.d.
n.d.
8.5
n.d.
Homodimer
Jia et al. (2000)
n.d., not determined
370
S. Ali and B. R. Glick