References
1. Karste K, Bleckmann M, van den Heuvel J
(2017) Not limited to E. coli: versatile expression vectors for mammalian protein expression.
Methods Mol Biol 1586:313–324
2. Meyer S, Lorenz C, Baser B et al (2013) Multihost expression system for recombinant production of challenging proteins. PLoS One 8
(7):e68674
3. Kamat V, Rafique A (2017) Designing binding
kinetic assay on the bio-layer interferometry
(BLI) biosensor to characterize antibodyantigen interactions. Anal Biochem 536:16–31
4. Egawa G, Kabashima K (2018) Barrier dysfunction in the skin allergy. Allergol Int
67:3–11
5. Sotiropoulou G, Pampalakis G (2012) Targeting the kallikrein-related peptidases for drug
development.
Trends
Pharmacol
Sci
33:623–634
6. Goettig P, Magdolen V, Brandstetter H (2010)
Natural and synthetic inhibitors of kallikreinrelated
peptidases
(klks).
Biochimie
92:1546–1567
7. Russo G, Meier D, Helmsing S et al (2018)
Parallelized antibody selection in microtiter
plates. Methods Mol Biol 1701:273–284
8. Frenzel A, Hust M, Schirrmann T (2016)
Phage display-derived antibodies in clinical
development and therapy. mAbs 8:1177–1194
9. Strohl WR (2018) Current progress in innovative engineered antibodies. Prot Cell 9:86–120
10. Frenzel A, Hust M, Schirrmann T (2013)
Expression of recombinant antibodies. Front
Immunol 4:217
11. Ha J-H, Kim J-E, Kim Y-S (2016) Immunoglobulin Fc heterodimer platform technology:
From design to applications in therapeutic antibodies and proteins. Front Immunol 7:394
12. Thie H, Toleikis L, Li J et al (2011) Rise and
fall of an anti-MUC1 specific antibody. PLoS
One 6:e15921
13. Wenzel EV, Bosnak M, Tierney R et al (2020)
Human antibodies neutralizing diphtheria
toxin in vitro and in vivo. Sci Rep 10:571
14. Li Z, Krippendorff B-F, Sharma S et al (2016)
Influence of molecular size on tissue distribution of antibody fragments. mAbs 8:113–119
15. Khan KH (2013) Gene expression in mammalian cells and its applications. Adv Pharm Bull
3:257–263
16. J€ ager V, Bu ¨ssow K, Wagner A et al (2013) High
level transient production of recombinant antibodies and antibody fusion proteins in
HEK293 cells. BMC Biotechnol 13:52
17. Li MZ, Elledge SJ (2012) SLIC: a method for
sequence- and ligation-independent cloning.
Methods Mol Biol 852:51–59
18. Fu ¨hner V, Heine PA, Helmsing S et al (2018)
Development
of
neutralizing
and
non-neutralizing antibodies targeting known
and novel epitopes of TCDB of Clostridioides
difficile. Front Microbiol 9:2908
19. Kumaraswamy S, Tobias R (2015) Label-Free
kinetic analysis of an antibody-antigen interaction using biolayer interferometry. Methods
Mol Biol 1278:165–182
20. Bleckmann M, Schu ¨rig M, Chen FF et al
(2016) Identification of essential genetic baculoviral elements for recombinant protein
expression by transactivation in Sf21 insect
cells. PloS One 11:e0149424
21. Shen X, Pitol AK, Bachmann V et al (2015) A
simple plasmid-based transient gene expression
method using High Five cells. J Biotechnol
216:67–75
22. Stolt-Bergner P, Benda C, Bergbrede T et al
(2018) Baculovirus-driven protein expression
in insect cells: a benchmarking study. J Struct
Biol 203:71–80
76
Jens Ko ¨ nig et al.
1. Karste K, Bleckmann M, van den Heuvel J
(2017) Not limited to E. coli: versatile expression vectors for mammalian protein expression.
Methods Mol Biol 1586:313–324
2. Meyer S, Lorenz C, Baser B et al (2013) Multihost expression system for recombinant production of challenging proteins. PLoS One 8
(7):e68674
3. Kamat V, Rafique A (2017) Designing binding
kinetic assay on the bio-layer interferometry
(BLI) biosensor to characterize antibodyantigen interactions. Anal Biochem 536:16–31
4. Egawa G, Kabashima K (2018) Barrier dysfunction in the skin allergy. Allergol Int
67:3–11
5. Sotiropoulou G, Pampalakis G (2012) Targeting the kallikrein-related peptidases for drug
development.
Trends
Pharmacol
Sci
33:623–634
6. Goettig P, Magdolen V, Brandstetter H (2010)
Natural and synthetic inhibitors of kallikreinrelated
peptidases
(klks).
Biochimie
92:1546–1567
7. Russo G, Meier D, Helmsing S et al (2018)
Parallelized antibody selection in microtiter
plates. Methods Mol Biol 1701:273–284
8. Frenzel A, Hust M, Schirrmann T (2016)
Phage display-derived antibodies in clinical
development and therapy. mAbs 8:1177–1194
9. Strohl WR (2018) Current progress in innovative engineered antibodies. Prot Cell 9:86–120
10. Frenzel A, Hust M, Schirrmann T (2013)
Expression of recombinant antibodies. Front
Immunol 4:217
11. Ha J-H, Kim J-E, Kim Y-S (2016) Immunoglobulin Fc heterodimer platform technology:
From design to applications in therapeutic antibodies and proteins. Front Immunol 7:394
12. Thie H, Toleikis L, Li J et al (2011) Rise and
fall of an anti-MUC1 specific antibody. PLoS
One 6:e15921
13. Wenzel EV, Bosnak M, Tierney R et al (2020)
Human antibodies neutralizing diphtheria
toxin in vitro and in vivo. Sci Rep 10:571
14. Li Z, Krippendorff B-F, Sharma S et al (2016)
Influence of molecular size on tissue distribution of antibody fragments. mAbs 8:113–119
15. Khan KH (2013) Gene expression in mammalian cells and its applications. Adv Pharm Bull
3:257–263
16. J€ ager V, Bu ¨ssow K, Wagner A et al (2013) High
level transient production of recombinant antibodies and antibody fusion proteins in
HEK293 cells. BMC Biotechnol 13:52
17. Li MZ, Elledge SJ (2012) SLIC: a method for
sequence- and ligation-independent cloning.
Methods Mol Biol 852:51–59
18. Fu ¨hner V, Heine PA, Helmsing S et al (2018)
Development
of
neutralizing
and
non-neutralizing antibodies targeting known
and novel epitopes of TCDB of Clostridioides
difficile. Front Microbiol 9:2908
19. Kumaraswamy S, Tobias R (2015) Label-Free
kinetic analysis of an antibody-antigen interaction using biolayer interferometry. Methods
Mol Biol 1278:165–182
20. Bleckmann M, Schu ¨rig M, Chen FF et al
(2016) Identification of essential genetic baculoviral elements for recombinant protein
expression by transactivation in Sf21 insect
cells. PloS One 11:e0149424
21. Shen X, Pitol AK, Bachmann V et al (2015) A
simple plasmid-based transient gene expression
method using High Five cells. J Biotechnol
216:67–75
22. Stolt-Bergner P, Benda C, Bergbrede T et al
(2018) Baculovirus-driven protein expression
in insect cells: a benchmarking study. J Struct
Biol 203:71–80
76
Jens Ko ¨ nig et al.