INDEX
A
Affimers................................................................. 105–120
Analytical ultracentrifugation (AUC) ................ 155, 157,
159, 161, 162, 167
Artificial binding proteins.................................... 105–120
B
Baculovirus insect cell expression................................... 18
BioID ................................................................... 304, 314,
316, 317
Biolayer interferometry (BLI) ..........................59, 61, 62,
64, 70, 74, 127
Biosensors ................................................................ 61, 65,
70, 75, 146
Biosimilars ....................................................................... 79
Biotin ................................................................... 111, 146,
304, 306, 309, 311, 314, 317
C
Chemical-crosslinking....................................75, 259, 260
Cloning .............................................................. 17–37, 45,
50, 55, 62, 63, 292, 294, 295, 299, 307, 308
Complex purification ........................................... 178, 303
Computational modelling .................................. 159, 222,
223, 233, 238
Confocal microscopy ........................................... 295, 296
CRISPR/Cas9.................................................. 39–56, 328
Cryo-electron microscopy (cryo-EM) ................. 78, 223,
224, 230, 244, 246–248, 253, 255, 271
Crystallography ..................................................... 77, 174,
223, 226
D
Detergents .........................................................6, 7, 9, 12,
13, 15, 137, 141, 188, 190, 215, 252, 253, 255,
257, 258, 262, 263, 266, 267
Directed evolution ............................................... 319–335
DNA ..................................................................18, 20, 21,
23–25, 27, 30, 32–37, 39–56, 62–65, 67, 73, 75,
85–88, 91, 93–96, 116, 125–141, 146, 148, 150,
180, 181, 193–217, 287, 291–295, 306–308,
319, 322, 326, 327, 329, 332, 335
DNA polymerase ...............................................23, 25, 30,
34, 42, 145–152
Double strand break (DSB) repair ........................ 44, 126
Double-stranded DNA donors ...................................... 40
DSTORM ............................................................. 271–284
E
EM sample preparation........................................ 244, 255
Endogenous host .............................................................. 7
Enzymes.............................................................21, 23, 24,
27, 35, 41, 47, 62, 147, 150, 178, 185, 199, 304,
306–308, 315, 316, 319, 320, 324, 330, 332, 334
Excited-state lifetime .................................. 288, 291, 298
Expression in bacteria ....................................................... 3
F
Fab .....................................................................60, 62, 70,
74, 75, 77–79, 89–102
Fluorescence ................................................. 6, 40, 43, 45,
54, 55, 91, 93, 128, 129, 131, 132, 135, 137,
138, 141, 145, 149, 150, 156, 168, 274–276,
279, 288, 291, 296, 307, 309, 310, 315, 316
Fluorescence imaging ............................. 54, 55, 291, 296
Fluorescence lifetime imaging microscopy
(FLIM)..............................................291, 295–298
Fluorescence microscopy ....................................... 52, 271
Fluorescent proteins..........................................6, 89, 101,
272, 291, 292, 296
Fo ¨rster resonance energy transfer
(FRET)..............................................288–291, 296
Functions ............................................................. 3, 39, 55,
60, 89, 91, 105, 131, 157, 160, 179, 207–209,
230, 232–234, 238, 243, 257, 263, 271, 278,
279, 281, 283, 287, 303, 320, 328
Fv fragments .................................................77–79, 84, 89
G
Genetically encoded fluorescent antibody ..................... 78
Genetic code expansion ................................................ 320
Genome editing .............................................................. 51
GFP-tag .............................................................. 43, 45, 52
H
Hetero-association ..............................156, 160, 163, 167
HIV reverse transcriptase..................................... 147, 148
Homology based cloning .........................................34, 55
Arnaud Poterszman (ed.), Multiprotein Complexes: Methods and Protocols, Methods in Molecular Biology, vol. 2247,
https://doi.org/10.1007/978-1-0716-1126-5, © Springer Science+Business Media, LLC, part of Springer Nature 2021
339
A
Affimers................................................................. 105–120
Analytical ultracentrifugation (AUC) ................ 155, 157,
159, 161, 162, 167
Artificial binding proteins.................................... 105–120
B
Baculovirus insect cell expression................................... 18
BioID ................................................................... 304, 314,
316, 317
Biolayer interferometry (BLI) ..........................59, 61, 62,
64, 70, 74, 127
Biosensors ................................................................ 61, 65,
70, 75, 146
Biosimilars ....................................................................... 79
Biotin ................................................................... 111, 146,
304, 306, 309, 311, 314, 317
C
Chemical-crosslinking....................................75, 259, 260
Cloning .............................................................. 17–37, 45,
50, 55, 62, 63, 292, 294, 295, 299, 307, 308
Complex purification ........................................... 178, 303
Computational modelling .................................. 159, 222,
223, 233, 238
Confocal microscopy ........................................... 295, 296
CRISPR/Cas9.................................................. 39–56, 328
Cryo-electron microscopy (cryo-EM) ................. 78, 223,
224, 230, 244, 246–248, 253, 255, 271
Crystallography ..................................................... 77, 174,
223, 226
D
Detergents .........................................................6, 7, 9, 12,
13, 15, 137, 141, 188, 190, 215, 252, 253, 255,
257, 258, 262, 263, 266, 267
Directed evolution ............................................... 319–335
DNA ..................................................................18, 20, 21,
23–25, 27, 30, 32–37, 39–56, 62–65, 67, 73, 75,
85–88, 91, 93–96, 116, 125–141, 146, 148, 150,
180, 181, 193–217, 287, 291–295, 306–308,
319, 322, 326, 327, 329, 332, 335
DNA polymerase ...............................................23, 25, 30,
34, 42, 145–152
Double strand break (DSB) repair ........................ 44, 126
Double-stranded DNA donors ...................................... 40
DSTORM ............................................................. 271–284
E
EM sample preparation........................................ 244, 255
Endogenous host .............................................................. 7
Enzymes.............................................................21, 23, 24,
27, 35, 41, 47, 62, 147, 150, 178, 185, 199, 304,
306–308, 315, 316, 319, 320, 324, 330, 332, 334
Excited-state lifetime .................................. 288, 291, 298
Expression in bacteria ....................................................... 3
F
Fab .....................................................................60, 62, 70,
74, 75, 77–79, 89–102
Fluorescence ................................................. 6, 40, 43, 45,
54, 55, 91, 93, 128, 129, 131, 132, 135, 137,
138, 141, 145, 149, 150, 156, 168, 274–276,
279, 288, 291, 296, 307, 309, 310, 315, 316
Fluorescence imaging ............................. 54, 55, 291, 296
Fluorescence lifetime imaging microscopy
(FLIM)..............................................291, 295–298
Fluorescence microscopy ....................................... 52, 271
Fluorescent proteins..........................................6, 89, 101,
272, 291, 292, 296
Fo ¨rster resonance energy transfer
(FRET)..............................................288–291, 296
Functions ............................................................. 3, 39, 55,
60, 89, 91, 105, 131, 157, 160, 179, 207–209,
230, 232–234, 238, 243, 257, 263, 271, 278,
279, 281, 283, 287, 303, 320, 328
Fv fragments .................................................77–79, 84, 89
G
Genetically encoded fluorescent antibody ..................... 78
Genetic code expansion ................................................ 320
Genome editing .............................................................. 51
GFP-tag .............................................................. 43, 45, 52
H
Hetero-association ..............................156, 160, 163, 167
HIV reverse transcriptase..................................... 147, 148
Homology based cloning .........................................34, 55
Arnaud Poterszman (ed.), Multiprotein Complexes: Methods and Protocols, Methods in Molecular Biology, vol. 2247,
https://doi.org/10.1007/978-1-0716-1126-5, © Springer Science+Business Media, LLC, part of Springer Nature 2021
339