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Landscape of nuclear transport receptor cargo
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15. Schopp IM, Be ´thune J (2018) Split-BioID—
proteomic analysis of context-specific protein
complexes in their native cellular environment.
J Vis Exp 134:57479. https://doi.org/10.
3791/57479
318
Cinthia Amaya Ramirez et al.
(2013) Novel bilobe components in Trypanosoma brucei identified using proximitydependent biotinylation. Eukaryot Cell 12
(2):356–367. https://doi.org/10.1128/EC.
00326-12
7. De Munter S, Gornemann J, Derua R et al
(2017) Split-BioID: a proximity biotinylation
assay for dimerization-dependent protein interactions. FEBS Lett 591(2):415–424. https://
doi.org/10.1002/1873-3468.12548
8. Schopp IM, Amaya Ramirez CC et al (2017)
Split-BioID a conditional proteomics approach
to monitor the composition of spatiotemporally defined protein complexes. Nat Commun
8:15690.
https://doi.org/10.1038/
ncomms15690
9. Opitz N, Schmitt K, Hofer-Pretz V et al (2017)
Capturing the Asc1p/receptor for activated C
kinase 1 (RACK1) microenvironment at the
head region of the 40S ribosome with quantitative BioID in yeast. Mol Cell Proteomics 16
(12):2199–2218. https://doi.org/10.1074/
mcp.M116.066654
10. Liu X, Salokas K, Tamene F et al (2018) An
AP-MS- and BioID-compatible MAC-tag
enables comprehensive mapping of protein
interactions and subcellular localizations. Nat
Commun 9(1):1188. https://doi.org/10.
1038/s41467-018-03523-2
11. Dyballa N, Metzger S (2009) Fast and sensitive
colloidal coomassie G-250 staining for proteins
in polyacrylamide gels. J Vis Exp 30. https://
doi.org/10.3791/1431
12. Kim DI, Cutler JA, Na CH et al (2018) BioSITe: a method for direct detection and quantitation of site-specific biotinylation. J
Proteome Res 17(2):759–769. https://doi.
org/10.1021/acs.jproteome.7b00775
13. Mackmull MT, Klaus B, Heinze I et al (2017)
Landscape of nuclear transport receptor cargo
specificity. Mol Syst Biol 13(12):962. https://
doi.org/10.15252/msb.20177608
14. Udeshi ND, Pedram K, Svinkina T et al (2017)
Antibodies to biotin enable large-scale detection of biotinylation sites on proteins. Nat
Methods 14(12):1167–1170. https://doi.
org/10.1038/nmeth.4465
15. Schopp IM, Be ´thune J (2018) Split-BioID—
proteomic analysis of context-specific protein
complexes in their native cellular environment.
J Vis Exp 134:57479. https://doi.org/10.
3791/57479
318
Cinthia Amaya Ramirez et al.