Fig. 1 Principle of split-BioID. Protein A interacts with protein B within Complex I or with protein C within
Complex II. Fusing the BirA* fragments NBirA* and CBirA* to protein A and protein B, respectively, leads to the
reassembly of an active enzyme upon dimerization of these two proteins and the specific labeling of further
components of complex
Fig. 2 Outline of the main steps in a typical split-BioID experiment
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Complex II. Fusing the BirA* fragments NBirA* and CBirA* to protein A and protein B, respectively, leads to the
reassembly of an active enzyme upon dimerization of these two proteins and the specific labeling of further
components of complex
Fig. 2 Outline of the main steps in a typical split-BioID experiment
Context-Specific Proximity-Dependent Proteomics with Split-BioID
305