9. Drew D, Lerch M, Kunji E et al (2006) Optimization of membrane protein overexpression
and purification using GFP fusions. Nat Methods 3:303–313
10. Kawate T, Gouaux E (2006) Fluorescencedetection size-exclusion chromatography for
precrystallization screening of integral membrane proteins. Structure 14:673–681
11. Duong F, Wickner W (1997) Distinct catalytic
roles of the SecYE, SecG and SecDFyajC subunits of preprotein translocase holoenzyme.
EMBO J 16:2756–2768
12. Scotti PA, Urbanus ML, Brunner J et al (2000)
YidC, the Escherichia coli homologue of mitochondrial Oxa1p, is a component of the Sec
translocase. EMBO J 19:542–549
13. Bieniossek C, Nie Y, Frey D et al (2009) Automated unrestricted multigene recombineering
for multiprotein complex production. Nat
Methods 6:447–450
14. Komar J, Botte M, Collinson I et al (2015)
ACEMBLing a multiprotein transmembrane
complex: the functional SecYEG-SecDF-YajCYidC Holotranslocon protein secretase/insertase. Methods Enzymol 556:23–49
15. Schulze RJ, Komar J, Botte M et al (2014)
Membrane protein insertion and protonmotive-force-dependent secretion through the
bacterial holo-translocon SecYEG-SecDFYajC-YidC. Proc Natl Acad Sci U S A
111:4844–4849
16. Botte M, Zaccai NR, Lycklama JA et al (2016)
A central cavity within the holo-translocon suggests a mechanism for membrane protein insertion. Sci Rep 6:38399
17. Thomas JA, Tate CG (2014) Quality control in
eukaryotic membrane protein overproduction.
J Mol Biol 426:4139–4154
18. Kost TA, Condreay JP, Jarvis DL (2005) Baculovirus as versatile vectors for protein
expression in insect and mammalian cells. Nat
Biotechnol 23:567–575
19. Akermoun M, Koglin M, Zvalova-Iooss D et al
(2005) Characterization of 16 human G
protein-coupled receptors expressed in
baculovirus-infected insect cells. Protein Expr
Purif 44:65–74
20. Rasmussen SG, Choi HJ, Rosenbaum DM et al
(2007) Crystal structure of the human beta2
adrenergic G-protein-coupled receptor. Nature
450:383–387
21. Fitzgerald DJ, Berger P, Schaffitzel C et al
(2006) Protein complex expression by using
multigene baculoviral vectors. Nat Methods
3:1021–1032
22. Khan I, Krishnaswamy S, Sabale M et al (2018)
Efficient production of a mature and functional
gamma secretase protease. Sci Rep 8:12834
23. Mesa P, Deniaud A, Montoya G et al (2013)
Directly from the source: endogenous preparations of molecular machines. Curr Opin Struct
Biol 23:319–325
24. Inoue H, Nojima H, Okayama H (1990) High
efficiency transformation of Escherichia coli
with plasmids. Gene 96:23–28
25. Dower WJ, Miller JF, Ragsdale CW (1988)
High efficiency transformation of E. coli by
high voltage electroporation. Nucleic Acids
Res 16:6127–6145
26. Linke D (2014) Explanatory chapter: choosing
the right detergent. Methods Enzymol
541:141–148
27. Kubicek J, Block H, Maertens B et al (2014)
Expression and purification of membrane proteins. Methods Enzymol 541:117–140
28. Lantez V, Nikolaidis I, Rechenmann M et al
(2015) Rapid automated detergent screening
for the solubilization and purification of membrane proteins and complexes. Eng Life Sci
15:39–50
16
Burak V. Kabasakal et al.
and purification using GFP fusions. Nat Methods 3:303–313
10. Kawate T, Gouaux E (2006) Fluorescencedetection size-exclusion chromatography for
precrystallization screening of integral membrane proteins. Structure 14:673–681
11. Duong F, Wickner W (1997) Distinct catalytic
roles of the SecYE, SecG and SecDFyajC subunits of preprotein translocase holoenzyme.
EMBO J 16:2756–2768
12. Scotti PA, Urbanus ML, Brunner J et al (2000)
YidC, the Escherichia coli homologue of mitochondrial Oxa1p, is a component of the Sec
translocase. EMBO J 19:542–549
13. Bieniossek C, Nie Y, Frey D et al (2009) Automated unrestricted multigene recombineering
for multiprotein complex production. Nat
Methods 6:447–450
14. Komar J, Botte M, Collinson I et al (2015)
ACEMBLing a multiprotein transmembrane
complex: the functional SecYEG-SecDF-YajCYidC Holotranslocon protein secretase/insertase. Methods Enzymol 556:23–49
15. Schulze RJ, Komar J, Botte M et al (2014)
Membrane protein insertion and protonmotive-force-dependent secretion through the
bacterial holo-translocon SecYEG-SecDFYajC-YidC. Proc Natl Acad Sci U S A
111:4844–4849
16. Botte M, Zaccai NR, Lycklama JA et al (2016)
A central cavity within the holo-translocon suggests a mechanism for membrane protein insertion. Sci Rep 6:38399
17. Thomas JA, Tate CG (2014) Quality control in
eukaryotic membrane protein overproduction.
J Mol Biol 426:4139–4154
18. Kost TA, Condreay JP, Jarvis DL (2005) Baculovirus as versatile vectors for protein
expression in insect and mammalian cells. Nat
Biotechnol 23:567–575
19. Akermoun M, Koglin M, Zvalova-Iooss D et al
(2005) Characterization of 16 human G
protein-coupled receptors expressed in
baculovirus-infected insect cells. Protein Expr
Purif 44:65–74
20. Rasmussen SG, Choi HJ, Rosenbaum DM et al
(2007) Crystal structure of the human beta2
adrenergic G-protein-coupled receptor. Nature
450:383–387
21. Fitzgerald DJ, Berger P, Schaffitzel C et al
(2006) Protein complex expression by using
multigene baculoviral vectors. Nat Methods
3:1021–1032
22. Khan I, Krishnaswamy S, Sabale M et al (2018)
Efficient production of a mature and functional
gamma secretase protease. Sci Rep 8:12834
23. Mesa P, Deniaud A, Montoya G et al (2013)
Directly from the source: endogenous preparations of molecular machines. Curr Opin Struct
Biol 23:319–325
24. Inoue H, Nojima H, Okayama H (1990) High
efficiency transformation of Escherichia coli
with plasmids. Gene 96:23–28
25. Dower WJ, Miller JF, Ragsdale CW (1988)
High efficiency transformation of E. coli by
high voltage electroporation. Nucleic Acids
Res 16:6127–6145
26. Linke D (2014) Explanatory chapter: choosing
the right detergent. Methods Enzymol
541:141–148
27. Kubicek J, Block H, Maertens B et al (2014)
Expression and purification of membrane proteins. Methods Enzymol 541:117–140
28. Lantez V, Nikolaidis I, Rechenmann M et al
(2015) Rapid automated detergent screening
for the solubilization and purification of membrane proteins and complexes. Eng Life Sci
15:39–50
16
Burak V. Kabasakal et al.