Acknowledgments
We acknowledge the support from the Institut National de la Sante ´
et de la Recherche Me ´dicale (INSERM), the Centre National pour
la Recherche Scientifique (CNRS), the Ligue contre le Cancer, and
the grant ANR-10-LABX-0030-INRT, a French State fund managed by the Agence Nationale de la Recherche under the frame
program Investissements d’Avenir ANR-10-IDEX-0002-02. We
acknowledge the use of resources of the French Infrastructure for
Integrated Structural Biology FRISBI ANR-10-INBS-05 and of
Instruct-ERIC.
References
1. Cheng Y (2018) Single-particle cryo-EM-how
did it get here and where will it go. Science 361
(6405):876–880
2. Dubochet J et al (1971) A new preparation
method for dark-field electron microscopy of
biomacromolecules. J Ultrastruct Res 35
(1):147–167
3. Brenner S, Horne RW (1959) A negative staining method for high resolution electron
microscopy of viruses. Biochim Biophys Acta
34:103–110
4. Jain T et al (2012) Spotiton: a prototype for an
integrated inkjet dispense and vitrification system for cryo-TEM. J Struct Biol 179(1):68–75
5. Dubochet J et al (1988) Cryo-electron microscopy of vitrified specimens. Q Rev Biophys 21
(2):129–228
6. Noble AJ et al (2018) Routine single particle
CryoEM sample and grid characterization by
tomography. Elife 7:e34257
7. Fukami A, Adachi K (1965) A new method of
preparation of a self-perforated micro plastic
grid and its application. J Electron Microsc 14
(2):112–118
8. Russo CJ, Passmore LA (2016) Progress
towards an optimal specimen support for electron cryomicroscopy. Curr Opin Struct Biol
37:81–89
9. Palovcak E et al (2018) A simple and robust
procedure for preparing graphene-oxide cryoEM grids. J Struct Biol 204(1):80–84
10. Vos MR et al (2008) The development of a
glove-box/Vitrobot combination: air-water
interface events visualized by cryo-TEM.
Ultramicroscopy 108(11):1478–1483
256
Alexandre Frechard et al.
We acknowledge the support from the Institut National de la Sante ´
et de la Recherche Me ´dicale (INSERM), the Centre National pour
la Recherche Scientifique (CNRS), the Ligue contre le Cancer, and
the grant ANR-10-LABX-0030-INRT, a French State fund managed by the Agence Nationale de la Recherche under the frame
program Investissements d’Avenir ANR-10-IDEX-0002-02. We
acknowledge the use of resources of the French Infrastructure for
Integrated Structural Biology FRISBI ANR-10-INBS-05 and of
Instruct-ERIC.
References
1. Cheng Y (2018) Single-particle cryo-EM-how
did it get here and where will it go. Science 361
(6405):876–880
2. Dubochet J et al (1971) A new preparation
method for dark-field electron microscopy of
biomacromolecules. J Ultrastruct Res 35
(1):147–167
3. Brenner S, Horne RW (1959) A negative staining method for high resolution electron
microscopy of viruses. Biochim Biophys Acta
34:103–110
4. Jain T et al (2012) Spotiton: a prototype for an
integrated inkjet dispense and vitrification system for cryo-TEM. J Struct Biol 179(1):68–75
5. Dubochet J et al (1988) Cryo-electron microscopy of vitrified specimens. Q Rev Biophys 21
(2):129–228
6. Noble AJ et al (2018) Routine single particle
CryoEM sample and grid characterization by
tomography. Elife 7:e34257
7. Fukami A, Adachi K (1965) A new method of
preparation of a self-perforated micro plastic
grid and its application. J Electron Microsc 14
(2):112–118
8. Russo CJ, Passmore LA (2016) Progress
towards an optimal specimen support for electron cryomicroscopy. Curr Opin Struct Biol
37:81–89
9. Palovcak E et al (2018) A simple and robust
procedure for preparing graphene-oxide cryoEM grids. J Struct Biol 204(1):80–84
10. Vos MR et al (2008) The development of a
glove-box/Vitrobot combination: air-water
interface events visualized by cryo-TEM.
Ultramicroscopy 108(11):1478–1483
256
Alexandre Frechard et al.