Khalid S, Stewart AG, Matthews SJ,
Gru ¨newald K, Robinson CV (2018) Protein
assemblies ejected directly from native membranes yield complexes for mass spectrometry.
Science 362(6416):829–834
14. Marcoux J, Robinson CV (2013) Twenty years
of gas phase structural biology. Structure 21
(9):1541–1550
15. Gan J, Ben-Nissan G, Arkind G, Tarnavsky M,
Trudeau D, Noda Garcia L, Tawfik DS, Sharon
M (2017) Native mass spectrometry of recombinant proteins from crude cell lysates. Anal
Chem 89(8):4398–4404
16. van de Waterbeemd M, Tamara S, Fort KL,
Damoc E, Franc V, Bieri P, Itten M,
Makarov A, Ban N, Heck AJR (2018) Dissecting ribosomal particles throughout the kingdoms of life using advanced hybrid mass
spectrometry methods. Nat Commun 9
(1):2493
17. Opalka N, Brown J, Lane WJ, Twist KA,
Landick R, Asturias FJ, Darst SA (2010) Complete structural model of Escherichia coli RNA
polymerase from a hybrid approach. PLoS Biol
8(9):e1000483
18. Twist KA, Husnain SI, Franke JD, Jain D,
Campbell EA, Nickels BE, Thomas MS, Darst
SA, Westblade LF (2011) A novel method for
the production of in vivo-assembled, recombinant Escherichia coli RNA polymerase lacking
the α C-terminal domain. Protein Sci 20
(6):986–995
19. Marty MT, Baldwin AJ, Marklund EG, Hochberg GK, Benesch JL, Robinson CV (2015)
Bayesian deconvolution of mass and ion mobility spectra: from binary interactions to polydisperse
ensembles.
Anal
Chem
87
(8):4370–4376
20. Reid DJ, Diesing JM, Miller MA, Perry SM,
Wales JA, Montfort WR, Marty MT (2019)
MetaUniDec: high-throughput deconvolution
of native mass spectra. J Am Soc Mass Spectrom 30(1):118–127
21. Radu L, Scho ¨nwetter E, Braun C, Marcoux J,
Ko ¨lmel W, Schmitt DR, Kuper J, Cianferani S,
Egly JM, Poterszman A, Kisker C (2017) The
intricate network between the p34 and p44
subunits is central to the activity of the transcription/DNA repair factor TFIIH. Nucleic
Acids Res 45(18):10872–10883
22. Kolesnikova O, Radu L, Poterszman A (2019)
TFIIH: a multi-subunit complex at the crossroads of transcription and DNA repair. Adv
Protein Chem Struct Biol 115:21–67
23. Fouillen L, Abdulrhaman W, Moras D, van
Dorsselaer A, Poterszman A, Sanglier-Cianfe ´rani S (2010) Analysis of recombinant phosphoprotein complexes with complementary mass
spectrometry approaches. Anal Biochem 407
(1):34–43
24. Mohideen-Abdul K, Tazibt K, Bourguet M,
Hazemann I, Lebars I, Takacs M,
Cianfe ´rani S, Klaholz BP, Moras D, Billas
IML (2017) Importance of the sequencedirected DNA shape for specific binding site
recognition by the estrogen-related receptor.
Front Endocrinol (Lausanne) 8:140
25. Horwich AL, Fenton WA, Chapman E, Farr
GW (2007) Two families of chaperonin: physiology and mechanism. Annu Rev Cell Dev Biol
23:115
26. Vitorino M, Coin F, Zlobinskaya O,
Atkinson
A,
Moras
D,
Egly
JM,
Poterszman A, Kieffer B (2007) Solution structure and self-association properties of the p8
TFIIH subunit responsible for Trichothiodystrophy. J Mol Biol 368(2):473–480
27. Raj SB, Ramaswamy S, Plapp BV (2014) Yeast
alcohol dehydrogenase structure and catalysis.
Biochemistry 53(36):5791–5803
28. Gervais V, Muller I, Mari PO, Mourcet A,
Movellan K, Ramos P, Marcoux J, Guillet V,
Javaid S, Burlet-Schiltz O, Czaplicki G,
Milon A, Giglia-Mari G (2018) Small
molecule-based targeting of TTD-A dimerization to control TFIIH transcriptional activity
represents a potential strategy for anticancer
therapy. J Biol Chem 293(39):14974–14988
29. Marcoux J, Wang S, Politis A, Reading E, Ma J,
Biggins P, Zhou M, Tao H, Zhang Q,
Chang G, Morgner N, Robinson CV (2013)
Mass spectrometry reveals synergistic binding
of nucleotides, lipids and drugs to a multidrug
resistance efflux pump. Proc Natl Acad Sci 110
(24):9704–9709
30. Laganowsky A, Reading E, Hopper JT, Robinson CV (2013) Mass spectrometry of intact
membrane protein complexes. Nat Protoc 8
(4):639–651
Native Mass Spectrometry
191
Gru ¨newald K, Robinson CV (2018) Protein
assemblies ejected directly from native membranes yield complexes for mass spectrometry.
Science 362(6416):829–834
14. Marcoux J, Robinson CV (2013) Twenty years
of gas phase structural biology. Structure 21
(9):1541–1550
15. Gan J, Ben-Nissan G, Arkind G, Tarnavsky M,
Trudeau D, Noda Garcia L, Tawfik DS, Sharon
M (2017) Native mass spectrometry of recombinant proteins from crude cell lysates. Anal
Chem 89(8):4398–4404
16. van de Waterbeemd M, Tamara S, Fort KL,
Damoc E, Franc V, Bieri P, Itten M,
Makarov A, Ban N, Heck AJR (2018) Dissecting ribosomal particles throughout the kingdoms of life using advanced hybrid mass
spectrometry methods. Nat Commun 9
(1):2493
17. Opalka N, Brown J, Lane WJ, Twist KA,
Landick R, Asturias FJ, Darst SA (2010) Complete structural model of Escherichia coli RNA
polymerase from a hybrid approach. PLoS Biol
8(9):e1000483
18. Twist KA, Husnain SI, Franke JD, Jain D,
Campbell EA, Nickels BE, Thomas MS, Darst
SA, Westblade LF (2011) A novel method for
the production of in vivo-assembled, recombinant Escherichia coli RNA polymerase lacking
the α C-terminal domain. Protein Sci 20
(6):986–995
19. Marty MT, Baldwin AJ, Marklund EG, Hochberg GK, Benesch JL, Robinson CV (2015)
Bayesian deconvolution of mass and ion mobility spectra: from binary interactions to polydisperse
ensembles.
Anal
Chem
87
(8):4370–4376
20. Reid DJ, Diesing JM, Miller MA, Perry SM,
Wales JA, Montfort WR, Marty MT (2019)
MetaUniDec: high-throughput deconvolution
of native mass spectra. J Am Soc Mass Spectrom 30(1):118–127
21. Radu L, Scho ¨nwetter E, Braun C, Marcoux J,
Ko ¨lmel W, Schmitt DR, Kuper J, Cianferani S,
Egly JM, Poterszman A, Kisker C (2017) The
intricate network between the p34 and p44
subunits is central to the activity of the transcription/DNA repair factor TFIIH. Nucleic
Acids Res 45(18):10872–10883
22. Kolesnikova O, Radu L, Poterszman A (2019)
TFIIH: a multi-subunit complex at the crossroads of transcription and DNA repair. Adv
Protein Chem Struct Biol 115:21–67
23. Fouillen L, Abdulrhaman W, Moras D, van
Dorsselaer A, Poterszman A, Sanglier-Cianfe ´rani S (2010) Analysis of recombinant phosphoprotein complexes with complementary mass
spectrometry approaches. Anal Biochem 407
(1):34–43
24. Mohideen-Abdul K, Tazibt K, Bourguet M,
Hazemann I, Lebars I, Takacs M,
Cianfe ´rani S, Klaholz BP, Moras D, Billas
IML (2017) Importance of the sequencedirected DNA shape for specific binding site
recognition by the estrogen-related receptor.
Front Endocrinol (Lausanne) 8:140
25. Horwich AL, Fenton WA, Chapman E, Farr
GW (2007) Two families of chaperonin: physiology and mechanism. Annu Rev Cell Dev Biol
23:115
26. Vitorino M, Coin F, Zlobinskaya O,
Atkinson
A,
Moras
D,
Egly
JM,
Poterszman A, Kieffer B (2007) Solution structure and self-association properties of the p8
TFIIH subunit responsible for Trichothiodystrophy. J Mol Biol 368(2):473–480
27. Raj SB, Ramaswamy S, Plapp BV (2014) Yeast
alcohol dehydrogenase structure and catalysis.
Biochemistry 53(36):5791–5803
28. Gervais V, Muller I, Mari PO, Mourcet A,
Movellan K, Ramos P, Marcoux J, Guillet V,
Javaid S, Burlet-Schiltz O, Czaplicki G,
Milon A, Giglia-Mari G (2018) Small
molecule-based targeting of TTD-A dimerization to control TFIIH transcriptional activity
represents a potential strategy for anticancer
therapy. J Biol Chem 293(39):14974–14988
29. Marcoux J, Wang S, Politis A, Reading E, Ma J,
Biggins P, Zhou M, Tao H, Zhang Q,
Chang G, Morgner N, Robinson CV (2013)
Mass spectrometry reveals synergistic binding
of nucleotides, lipids and drugs to a multidrug
resistance efflux pump. Proc Natl Acad Sci 110
(24):9704–9709
30. Laganowsky A, Reading E, Hopper JT, Robinson CV (2013) Mass spectrometry of intact
membrane protein complexes. Nat Protoc 8
(4):639–651
Native Mass Spectrometry
191