3.4.2 Q-TOF Synapt G2
and G2Si
1. Using the TriVersa Nanomate™ (see Subheading 3.3), deliver
5 μL of cesium iodide calibration mix (see Subheading 2.2).
2. In MassLynx MS Tune windows, manually set the following
parameters: Operate the instrument in positive and sensitivity
modes with an m/z range from 1,000 to 8,000.
3. Nanoflow+ panel: Sampling Cone: 150 V. Source Offset: 30 V.
Source Temperature: 80
C. The Capillary Voltage and the gas
flows are not used in the TriVersa Nanomate™ interface setup.
4. Instrument: Trap and Transfer Collision Energy are turned off
(4 V and 2 V, respectively, by default).
5. Save these parameters as an .ipr file.
6. Press “acquire” to start a 2-min TOF-MS acquisition in the
1,000–8,000 m/z range.
7. Average the signal over the 2 min in the Chromatogram panel
and in the Spectrum panel, select Process\Automatic Peak
Detection with the “Set Peak Detection Parameters Automatically” option activated.
8. Click on File\Save Spectrum and click OK.
9. In the Acquity UPLC Console, click on Intellistart, select
“Create Calibration,” and click Start.
10. In the calibration profile editor, select the right Mass Calibration Profile (here CsI in the 1,000–8,000 m/z range), right
click, and reset it.
11. Right Click and edit the Mass Calibration Profile. Below the
Positive Polarity chart, click on edit. Select the right Reference
Compound (here CsI_1,000–8,000 (positive) with reference
masses ranging from 1,172.1450 Da to 7,927.2031 Da) (see
Note 7).
12. Browse to select the previous acquisition file. Click on History
and select the last AccMass2 line.
13. Close the four windows by clicking OK and close the Mass
Calibration Profile Editor.
14. Click Next, Next, and Start to launch the calibration.
3.4.3 Q-TOF Maxis II
1. Using the HESI source and the 500 μL Hamilton™ syringe,
deliver cesium iodide calibration mix (see Subheading 2.2) with
a flow rate of 3 μL/min.
2. In the MS Tune window, manually set the following
parameters:
Operate the instrument in positive mode with an m/z
range from 1,000 to 8,000. Apply 20 eV and 5 eV for is CID
and CE parameters, respectively. Furthermore, native experiments involve adapting transfer and prepulse storage times (see
Note 8): set to 320 μs and 15 μs, respectively. Concerning the
Native Mass Spectrometry
177
and G2Si
1. Using the TriVersa Nanomate™ (see Subheading 3.3), deliver
5 μL of cesium iodide calibration mix (see Subheading 2.2).
2. In MassLynx MS Tune windows, manually set the following
parameters: Operate the instrument in positive and sensitivity
modes with an m/z range from 1,000 to 8,000.
3. Nanoflow+ panel: Sampling Cone: 150 V. Source Offset: 30 V.
Source Temperature: 80
C. The Capillary Voltage and the gas
flows are not used in the TriVersa Nanomate™ interface setup.
4. Instrument: Trap and Transfer Collision Energy are turned off
(4 V and 2 V, respectively, by default).
5. Save these parameters as an .ipr file.
6. Press “acquire” to start a 2-min TOF-MS acquisition in the
1,000–8,000 m/z range.
7. Average the signal over the 2 min in the Chromatogram panel
and in the Spectrum panel, select Process\Automatic Peak
Detection with the “Set Peak Detection Parameters Automatically” option activated.
8. Click on File\Save Spectrum and click OK.
9. In the Acquity UPLC Console, click on Intellistart, select
“Create Calibration,” and click Start.
10. In the calibration profile editor, select the right Mass Calibration Profile (here CsI in the 1,000–8,000 m/z range), right
click, and reset it.
11. Right Click and edit the Mass Calibration Profile. Below the
Positive Polarity chart, click on edit. Select the right Reference
Compound (here CsI_1,000–8,000 (positive) with reference
masses ranging from 1,172.1450 Da to 7,927.2031 Da) (see
Note 7).
12. Browse to select the previous acquisition file. Click on History
and select the last AccMass2 line.
13. Close the four windows by clicking OK and close the Mass
Calibration Profile Editor.
14. Click Next, Next, and Start to launch the calibration.
3.4.3 Q-TOF Maxis II
1. Using the HESI source and the 500 μL Hamilton™ syringe,
deliver cesium iodide calibration mix (see Subheading 2.2) with
a flow rate of 3 μL/min.
2. In the MS Tune window, manually set the following
parameters:
Operate the instrument in positive mode with an m/z
range from 1,000 to 8,000. Apply 20 eV and 5 eV for is CID
and CE parameters, respectively. Furthermore, native experiments involve adapting transfer and prepulse storage times (see
Note 8): set to 320 μs and 15 μs, respectively. Concerning the
Native Mass Spectrometry
177