11. Ethanol absolute 99.9%.
12. 1.5 and 10 mL autosampler vials with Septa caps and insets.
2.2 Enzyme
Preparation
1. Reverse transcriptase HIV-1, BH10 isolate (RT) was expressed
and purified as described previously [15] and stored as a suspension in a 2 M (NH 4 ) 2 SO 4 solution.
Fig. 1 Schematic representation of the HIV-1 DNA polymerase switchSENSE experiment. (a) Illustration
showing the building of the DNA nanolever following by regeneration of the biosensor chip. (b) Scheme of
the switchSENSE measurement described here, containing three main steps: association, polymerization, and
dissociation
Analysis of a DNA Polymerase Using switchSENSE Approach
147
12. 1.5 and 10 mL autosampler vials with Septa caps and insets.
2.2 Enzyme
Preparation
1. Reverse transcriptase HIV-1, BH10 isolate (RT) was expressed
and purified as described previously [15] and stored as a suspension in a 2 M (NH 4 ) 2 SO 4 solution.
Fig. 1 Schematic representation of the HIV-1 DNA polymerase switchSENSE experiment. (a) Illustration
showing the building of the DNA nanolever following by regeneration of the biosensor chip. (b) Scheme of
the switchSENSE measurement described here, containing three main steps: association, polymerization, and
dissociation
Analysis of a DNA Polymerase Using switchSENSE Approach
147