Fig. 4 Structure of the Bac-to-Bac entry vector pFBgp67-iRATmC for the iRAT-based Fab expression. (a)
Schematic illustration of the region containing the promoter, secretion signal, and iRAT-polyprotein construct.
(b) Universal PCR primers for construction of expression plasmid. (c) Sequence corresponding to the region
shown in (a). The gp67 secretion signal, TEV recognition sequence, His 6 tag, and mCherry are shown in gray,
orange, yellow, and purple, respectively. Gray arrowheads indicate the signal cleavage site and TEV cleavage
sites. The sc70-derived linker regions are shown in green
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Norimichi Nomura et al.
Schematic illustration of the region containing the promoter, secretion signal, and iRAT-polyprotein construct.
(b) Universal PCR primers for construction of expression plasmid. (c) Sequence corresponding to the region
shown in (a). The gp67 secretion signal, TEV recognition sequence, His 6 tag, and mCherry are shown in gray,
orange, yellow, and purple, respectively. Gray arrowheads indicate the signal cleavage site and TEV cleavage
sites. The sc70-derived linker regions are shown in green
92
Norimichi Nomura et al.