Table 15.1 Summary of genetic transformation of duckweeds
Species
Stable/Transient
Transformation
method
Gene and gene
function
Selectable marker
References
Lemna
minor
Stable
Lemna
Expression
System (LEX
SystemSM,
Biolex
Therapeutics,
Pittsboro, NC)
Hemagglutinin (HA)
gene, against
H5N1 HPAI viruses
Kanamycin
Bertran
et al.
(2015)
Agrobacterium
tumefaciens
(GV3101)
gfp gene, reporter
gene
Phosphinothricin
(PPT)
CantóPastor et al.
(2015)
Agrobacterium
tumefaciens
(CBE21)
M130-bglucuronidase gene
Kanamycin
Firsov et al.
(2015)
Agrobacterium
tumefaciens
(C58-z707)
Avian influenza
hemagglutinin HA
gene, against
H5N1 HPAI viruses;
threonine deaminase
(TD) gene,
expressing threonine
deaminase
Kanamycin
Long et al.
(2012)
Agrobacterium
tumefaciens
(EHA105)
Glyoxylate
aminotransferase
(SGAT) AtAGT1,
involving in
photorespiratory
pathway in
resistance to salt
stress
Hygromycin
Yang, et al.
(2013)
Agrobacterium
tumefaciens
(EHA105)
Porcine epidemic
diarrhea virus
(PEDV) spike
protein-1 gene,
producing a
protective antigen
for the PEDV spike
protein 1
Kanamycin
Ko et al.
(2011)
Agrobacterium
tumefaciens
(C58-Z707)
uidA gene, reporter
gene
Kanamycin
Yamamoto
et al.
(2001)
Agrobacterium
tumefaciens
(C58-z707)
E1 endoglucanase
gene, expressing E1
protein
Kanamycin
Sun et al.
(2007)
Agrobacterium
tumefaciens
(C58-z707)
Human monoclonal
antibodies (mAbs)
MDX-060 synthetic
gene, an anti-CD30
antibody for the
Kanamycin
Cox et al.
(2006)
(continued)
15 Transformation Development in Duckweeds
145