11
Genotyping-by-Sequencing
for Species Delimitation in Lemna
Section Uninerves Hegelm.
(Lemnaceae)
M. Bog, S. Xu, A. Himmelbach, R. Brandt, F. Wagner,
K.-J. Appenroth and K. S. Sree
Abstract
Lemna section Uninerves presently consists of
three species, Lemna minuta Kunth (common
synonyms L. minima Chev., L. minuscula
Herter), L. valdiviana Phil., and L. yungensis
Landolt. The species L. yungensis was discovered by E. Landolt about 20 years ago. He
mentioned that although this species specifically grows on wet rocks, it is closely related
to L. valdiviana and is morphologically very
similar to the other two species in this section.
Therefore, we started with molecular taxonomic studies of this section by using
genotyping-by-sequencing (GBS). It was
found that L. minuta could be clearly separated from L. valdiviana/L. yungensis; however, no separation was found between
L. valdiviana and L. yungensis, despite the
fact that they occupy different ecological
niches. These data revealed that L. yungensis
is identical to L. valdiviana on the genetic
basis. Based on these results, we suggest that
the name Lemna yungensis should be synonymised. As the description of L. valdiviana
Phil. is the older one, this would be the valid
common name for all the clones defined
before as L. valdiviana and L. yungensis. This
decision reduces the number of Lemnaceae
species to 36. Testing the method of GBS with
the genus Lemna demonstrated its superiority
in comparison with barcoding based on PCR
amplifications.
11.1 Introduction
Lemna section Uninerves Hegelm. represents the
most reduced group, based on morphology,
within the genus Lemna L. (Lemnaceae) positioning it as the most derived one on evolutionary
basis (Landolt 1986; Les et al. 1997). After the
revision of the internal structure of Lemna by Les
et al. (2002), all remaining sections are monophyletic, i.e. L. section Lemna, L. section Alatae,
L. section Biformes, and L. section Uninerves, each with 100% bootstrap support. Lemna
M. Bog (&)
Institute of Botany and Landscape Ecology,
University of Greifswald, Greifswald, Germany
e-mail: manuela.bog@uni-greifswald.de
S. Xu
Institute for Evolution and Biodiversity,
University of Münster, Münster, Germany
A. Himmelbach Á R. Brandt
Leibniz Institute of Plant Genetics and Crop Plant
Research (IPK), Gatersleben, Germany
F. Wagner
Institute of Plant Sciences, University
of Regensburg, Regensburg, Germany
K.-J. Appenroth
University of Jena, Matthias Schleiden Institute,
Plant Physiology, Jena, Germany
K. S. Sree
Department of Environmental Science,
Central University of Kerala, Periye, India
© Springer Nature Switzerland AG 2020
X. H. Cao et al. (eds.), The Duckweed Genomes, Compendium of Plant Genomes,
https://doi.org/10.1007/978-3-030-11045-1_11
115
Genotyping-by-Sequencing
for Species Delimitation in Lemna
Section Uninerves Hegelm.
(Lemnaceae)
M. Bog, S. Xu, A. Himmelbach, R. Brandt, F. Wagner,
K.-J. Appenroth and K. S. Sree
Abstract
Lemna section Uninerves presently consists of
three species, Lemna minuta Kunth (common
synonyms L. minima Chev., L. minuscula
Herter), L. valdiviana Phil., and L. yungensis
Landolt. The species L. yungensis was discovered by E. Landolt about 20 years ago. He
mentioned that although this species specifically grows on wet rocks, it is closely related
to L. valdiviana and is morphologically very
similar to the other two species in this section.
Therefore, we started with molecular taxonomic studies of this section by using
genotyping-by-sequencing (GBS). It was
found that L. minuta could be clearly separated from L. valdiviana/L. yungensis; however, no separation was found between
L. valdiviana and L. yungensis, despite the
fact that they occupy different ecological
niches. These data revealed that L. yungensis
is identical to L. valdiviana on the genetic
basis. Based on these results, we suggest that
the name Lemna yungensis should be synonymised. As the description of L. valdiviana
Phil. is the older one, this would be the valid
common name for all the clones defined
before as L. valdiviana and L. yungensis. This
decision reduces the number of Lemnaceae
species to 36. Testing the method of GBS with
the genus Lemna demonstrated its superiority
in comparison with barcoding based on PCR
amplifications.
11.1 Introduction
Lemna section Uninerves Hegelm. represents the
most reduced group, based on morphology,
within the genus Lemna L. (Lemnaceae) positioning it as the most derived one on evolutionary
basis (Landolt 1986; Les et al. 1997). After the
revision of the internal structure of Lemna by Les
et al. (2002), all remaining sections are monophyletic, i.e. L. section Lemna, L. section Alatae,
L. section Biformes, and L. section Uninerves, each with 100% bootstrap support. Lemna
M. Bog (&)
Institute of Botany and Landscape Ecology,
University of Greifswald, Greifswald, Germany
e-mail: manuela.bog@uni-greifswald.de
S. Xu
Institute for Evolution and Biodiversity,
University of Münster, Münster, Germany
A. Himmelbach Á R. Brandt
Leibniz Institute of Plant Genetics and Crop Plant
Research (IPK), Gatersleben, Germany
F. Wagner
Institute of Plant Sciences, University
of Regensburg, Regensburg, Germany
K.-J. Appenroth
University of Jena, Matthias Schleiden Institute,
Plant Physiology, Jena, Germany
K. S. Sree
Department of Environmental Science,
Central University of Kerala, Periye, India
© Springer Nature Switzerland AG 2020
X. H. Cao et al. (eds.), The Duckweed Genomes, Compendium of Plant Genomes,
https://doi.org/10.1007/978-3-030-11045-1_11
115