194
Stroke
neurovascular damage, 178
VEGF, 179, 180
Stromal cell-derived factor 1 (SDF-1), 160
Sun, J., 40, 41
T
Tan, Y., 134
3D culture systems, vascular networks, 38
3D printing methods
advantages, 135
application, 136
bioinks, 135
biomaterials, 136
cell-cell signaling, 136
clinical goal, 137
computational analysis, 137
cytocompatibility, 136
extrusion-based printing (see Extrusionbased printing)
fabrication, vascular tissue, 136
inkjet-based printing (see Inkjet-based
printing)
internal structure, 121
laser-based 3D printing (see Laser-based
3D printing)
material behavior, 121
maturogenic factors, 136
microvascular network creation, 123
nozzle-free approach, 135
scaffold-free 3D printing (see Scaffold-free
3D printing)
tissue engineering, 121
vascular constructs, 121, 135
vascular graft creation, 124
Tissue engineering
brain angiogenesis, 180–182, 184
Tissue inhibitor of metalloproteinase (TIMP),
13, 14, 18
Tomé, I., 51–66
Twardowski, R.L., 151
U
Ushio-Fukai, M., 80
V
Vascular aging
age-related biological changes, 51
chronic diseases, 51
DNA damage, 52
ECM, 66
environmental stresses, 52
general insights, 52, 53
HGPS (see Hutchinson-Gilford Progeria
syndrome (HGPS))
Hutchinson-Gilford progeria syndrome, 52
in vitro systems, 62–64
iPSCs, 65
mechanosensitivity, 66
p53/p21 and p16
INK4a
/pRB pathways, 52
physiological
biophysical changes, 53
ECM remodeling, 53–55
enhanced fibrosis, 55
flow shear stress, 57
and HGPS, 59
vascular cell dysfunction, 55–57
proteostasis, 52
and senescence, 51
Vascular endothelial growth factor (VEGF),
43, 44, 150, 160, 163
endogenous angiogenesis, 179
HA-VEGF nanocapsule delivery, 183
Laminin-VEGF transplantation, 182
PDMS-TEOS material, 181
therapeutic approach, 180
Vascular morphogenesis
extracellular matrix, 2, 3
MT1-MMP controls, 13
Vascular networks
endothelial progenitor cells, 37
fibrin concentrations, 39
formation and maturation, 45
formation of, 38
in 3D engineered tissue, 44
vasculogenesis, 45
Vascular smooth muscle cells (vSMC), 41
Vascular tissue engineering, 137
Vascularization of 3D-engineered tissues
angiogenesis, 37
anti-angiogenic role, 45
cell-induced contractile forces, 45
experimental approaches, 45
external forces
fluid shear stress, 42–44
tensile, 41, 43
high-precision fabrication techniques, 45
internal and external mechanical cues, 38, 39
internal forces
boundary constraint, 40
cellular, 38
matrix stiffness, 39, 40
matrix stiffness, 38
Index
Stroke
neurovascular damage, 178
VEGF, 179, 180
Stromal cell-derived factor 1 (SDF-1), 160
Sun, J., 40, 41
T
Tan, Y., 134
3D culture systems, vascular networks, 38
3D printing methods
advantages, 135
application, 136
bioinks, 135
biomaterials, 136
cell-cell signaling, 136
clinical goal, 137
computational analysis, 137
cytocompatibility, 136
extrusion-based printing (see Extrusionbased printing)
fabrication, vascular tissue, 136
inkjet-based printing (see Inkjet-based
printing)
internal structure, 121
laser-based 3D printing (see Laser-based
3D printing)
material behavior, 121
maturogenic factors, 136
microvascular network creation, 123
nozzle-free approach, 135
scaffold-free 3D printing (see Scaffold-free
3D printing)
tissue engineering, 121
vascular constructs, 121, 135
vascular graft creation, 124
Tissue engineering
brain angiogenesis, 180–182, 184
Tissue inhibitor of metalloproteinase (TIMP),
13, 14, 18
Tomé, I., 51–66
Twardowski, R.L., 151
U
Ushio-Fukai, M., 80
V
Vascular aging
age-related biological changes, 51
chronic diseases, 51
DNA damage, 52
ECM, 66
environmental stresses, 52
general insights, 52, 53
HGPS (see Hutchinson-Gilford Progeria
syndrome (HGPS))
Hutchinson-Gilford progeria syndrome, 52
in vitro systems, 62–64
iPSCs, 65
mechanosensitivity, 66
p53/p21 and p16
INK4a
/pRB pathways, 52
physiological
biophysical changes, 53
ECM remodeling, 53–55
enhanced fibrosis, 55
flow shear stress, 57
and HGPS, 59
vascular cell dysfunction, 55–57
proteostasis, 52
and senescence, 51
Vascular endothelial growth factor (VEGF),
43, 44, 150, 160, 163
endogenous angiogenesis, 179
HA-VEGF nanocapsule delivery, 183
Laminin-VEGF transplantation, 182
PDMS-TEOS material, 181
therapeutic approach, 180
Vascular morphogenesis
extracellular matrix, 2, 3
MT1-MMP controls, 13
Vascular networks
endothelial progenitor cells, 37
fibrin concentrations, 39
formation and maturation, 45
formation of, 38
in 3D engineered tissue, 44
vasculogenesis, 45
Vascular smooth muscle cells (vSMC), 41
Vascular tissue engineering, 137
Vascularization of 3D-engineered tissues
angiogenesis, 37
anti-angiogenic role, 45
cell-induced contractile forces, 45
experimental approaches, 45
external forces
fluid shear stress, 42–44
tensile, 41, 43
high-precision fabrication techniques, 45
internal and external mechanical cues, 38, 39
internal forces
boundary constraint, 40
cellular, 38
matrix stiffness, 39, 40
matrix stiffness, 38
Index