1.10 Post-reconstruction Image Processing
This section introduces the strategies for mining the complexity of tomograms,
which are covered in detail in subsequent chapters (Chaps. 8, 11 and 12). These
optional techniques are segmentation, which is often aided by prior denoising, and
subtomogram averaging. Template matching can be used to select subtomographic
volumes for subtomogram averaging; this can be regarded as a special form of
segmentation.
Fig. 1.6 Progress in segmentation. Early 1970’s. a Thin section electron micrograph of a
compartmentalised bacterium and b ‘artist’s impression’ of internal structure based on the
micrograph and a clay model [104]; c surface-rendering of an appendage from a mammalian cell
grown in tissue culture
18
A. Leis
This section introduces the strategies for mining the complexity of tomograms,
which are covered in detail in subsequent chapters (Chaps. 8, 11 and 12). These
optional techniques are segmentation, which is often aided by prior denoising, and
subtomogram averaging. Template matching can be used to select subtomographic
volumes for subtomogram averaging; this can be regarded as a special form of
segmentation.
Fig. 1.6 Progress in segmentation. Early 1970’s. a Thin section electron micrograph of a
compartmentalised bacterium and b ‘artist’s impression’ of internal structure based on the
micrograph and a clay model [104]; c surface-rendering of an appendage from a mammalian cell
grown in tissue culture
18
A. Leis