G
GABA, 164
Gaussian filter, 288, 290, 291, 293–295
Geometrical beam divergence, 40
Geometry, 302
Geometry of acquisition, 266
Glutaraldehyde, 155
Gold fiducial markers, 66
Gold standard alignment’, 244
Goniometer, 24
Gridded-based interpolation, 214
H
Heterogeneity, 246, 277
Hidden variables, 242
High-Angle Annular DF (HAADF), 34
High-Angle Annular DF (HAADF) detectors,
38, 45
Higher quality images, 85
Higher resolution, 85
Higher voltage, 73
High-pressures, 67
Human intervention, 311
Hybrid method, 278
Hybrid techniques, 3
I
Ice/sample thickness, 269
Identify structures, 80
Image convolution, 286, 288, 291
Image registration, 159
Immunoelectron microscopy, 149
Immunogold labeling, 151
Immunostaining, 156
Improved algorithms, 86
Improved imaging, 85
Incident beam convergence, 41
Inelastically scattered, 11
Inelastic and multiple scattering, 67
Inelastic scattering, 36
Influenza virus, 286, 288, 293
Information yield, 22
In situ, 61
Interpolation, 210, 213, 217, 278
Interpret, 86
Isosurfaces, 79
Iterative median filter, 224
Iterative optimization, 243
Iterative reconstruction, 214
Iterative reconstruction algorithms, 16
J
Job file, 252, 254, 255
L
Light elements, 39
Limit of sample thickness, 38
Linear diffusion, 290, 291
Lipid droplets, 49
Liquid cryogen, 66
Liquid nitrogen, 67
Loading, 68
Load specimen, 88
Local cross-correlation, 276
Localization accuracy, 307
Local resolution, 245
Longitudinal beam profiles, 40
Low atomic number Z, 46
Low dose, 71
Lowicryl HM20, 152, 156
Low-pass filter, 224
Low signal-to-noise, 64
Lung fibroblast cells, 49
M
Machine learning, 312
Marker-free alignment, 14
Mask, 252
Masking, 78
Mass density variation, 46
Mass loss, 11
Mass measurements, 44
Mass/thickness contrast, 39
Matched filter, 307
Maximum entropy method, 216
Maximum likelihood, 242
Mean Free Path (MFP), 38
Mean shift filtering, 225
Mechanistic insights, 82
Median filter, 289, 290
MFP for inelastic scattering by vitreous ice, 38
M-free score, 307
Microtrabecular lattice, 7
Microtubule, 49, 250
Missing information, 211, 226
Missing pyramid, 24
Missing wedge, 24, 65, 75, 266, 302
Mitochondria, 49
Mitochondrion, 290
Model-based averaging, 230
Model-based methods, 304
Index
321
GABA, 164
Gaussian filter, 288, 290, 291, 293–295
Geometrical beam divergence, 40
Geometry, 302
Geometry of acquisition, 266
Glutaraldehyde, 155
Gold fiducial markers, 66
Gold standard alignment’, 244
Goniometer, 24
Gridded-based interpolation, 214
H
Heterogeneity, 246, 277
Hidden variables, 242
High-Angle Annular DF (HAADF), 34
High-Angle Annular DF (HAADF) detectors,
38, 45
Higher quality images, 85
Higher resolution, 85
Higher voltage, 73
High-pressures, 67
Human intervention, 311
Hybrid method, 278
Hybrid techniques, 3
I
Ice/sample thickness, 269
Identify structures, 80
Image convolution, 286, 288, 291
Image registration, 159
Immunoelectron microscopy, 149
Immunogold labeling, 151
Immunostaining, 156
Improved algorithms, 86
Improved imaging, 85
Incident beam convergence, 41
Inelastically scattered, 11
Inelastic and multiple scattering, 67
Inelastic scattering, 36
Influenza virus, 286, 288, 293
Information yield, 22
In situ, 61
Interpolation, 210, 213, 217, 278
Interpret, 86
Isosurfaces, 79
Iterative median filter, 224
Iterative optimization, 243
Iterative reconstruction, 214
Iterative reconstruction algorithms, 16
J
Job file, 252, 254, 255
L
Light elements, 39
Limit of sample thickness, 38
Linear diffusion, 290, 291
Lipid droplets, 49
Liquid cryogen, 66
Liquid nitrogen, 67
Loading, 68
Load specimen, 88
Local cross-correlation, 276
Localization accuracy, 307
Local resolution, 245
Longitudinal beam profiles, 40
Low atomic number Z, 46
Low dose, 71
Lowicryl HM20, 152, 156
Low-pass filter, 224
Low signal-to-noise, 64
Lung fibroblast cells, 49
M
Machine learning, 312
Marker-free alignment, 14
Mask, 252
Masking, 78
Mass density variation, 46
Mass loss, 11
Mass measurements, 44
Mass/thickness contrast, 39
Matched filter, 307
Maximum entropy method, 216
Maximum likelihood, 242
Mean Free Path (MFP), 38
Mean shift filtering, 225
Mechanistic insights, 82
Median filter, 289, 290
MFP for inelastic scattering by vitreous ice, 38
M-free score, 307
Microtrabecular lattice, 7
Microtubule, 49, 250
Missing information, 211, 226
Missing pyramid, 24
Missing wedge, 24, 65, 75, 266, 302
Mitochondria, 49
Mitochondrion, 290
Model-based averaging, 230
Model-based methods, 304
Index
321