Table 10.1
Summary of the selected high-resolution structures determined by StA
Object
Microscope Camera
Tilt range and
step
Pixel
size
Number of
asym units
Underfocus
range, microns
Electron
dose,
e
−
/Å
2
Resolution,
Å
Reference
Isolated GroEL
FEI Titan
Krios
4 k CCD @
80 kV
−45:2:56
1.74
*70,000
2…3
106
8.4
[61]
GAG, tubular
crystal
FEI Titan
Krios
2 k CCD
@200 kV + GIF
−45:3:60
2.44
*243,000
1.5…3.3
40
8.5
[47]
GAG in native HIV
virions
FEI Titan
Krios
2 k CCD
@200 kV + GIF
−45:3:60
2
*200,000
1.2…4
40
8.8
[59]
GAG in RSV
virions
FEI Titan
Krios
2kCCD
@200 kV + GIF
−45:3:45 or
−30:3:30
1
*50,000
1.5…5
2 4
…36
7.7
[62]
HBV capsid
FEI Titan
Krios
K2
Summit + GIF
−60:3:60
2.17
*68,000
3.3…5.6
60
8.1
[63]
Ribosome-sec61
FEI Titan
Krios
K2 Summit
−60:2:60
2.62
17,600
3…4
9 0
…100* 9
[60]
Ion channel 5HT-3
in lipid vesicles
FEI Titan
Krios
K2 Summit
−60:3:60
1.67
*65,000
2.5
… 4
4 1
1 2
[ 64]
Tobacco Mosaic
Virus
FEI Titan
Krios
K2
Summit + GIF
−60:3:60
1.1
3625
2
80
8.5
[65]
GAG in native HIV
virions
FEI Titan
Krios
K2
Summit + GIF
0,−3, 3, 6,
−6,
−9… [66]
1.3
*600,000
1.5
… 5
9 0
…150
3.9
[67]
274
M. Kudryashev