diagnostics, this chapter deals with the potential of impedimetric aptasensors for
aptamer-based cancer treatment by oncolytic virus.
2.2.1 Cancer Cells
The state-of-the-art diagnostics of cancer types that are based on imaging methods
are limited since they mainly analyze morphology. However, morphology-based
classification does not allow for early-stage-diagnosis as morphologic changes do
not occur right away. As an alternative, impedimetric aptasensors are an opportunity
of rapid and label-free detection of cancer cells themselves. For instance, CT26
(colorectal cancer) were detected by an impedimetric aptasensor as a rapid and labelfree approach [39]. The proposed sensor enabled LoD of 2 cells/ml and a linear range
of 1 Â 10
5 –6 Â 10
6 cells/ml.
Diagnosis of hepatocellular carcinoma (HCC) goes along with poor prognosis for
survival the next 5 years due to the lack of effective treatment strategies. So far, the
Fig. 3 Gold electrodes are often functionalized by well-known gold sulfur chemistry. In order to
achieve a self-assembled monolayer (SAM) and to reduce non-specific binding, the application of
short backfillers is a common strategy besides co-immobilization or post-functionalization of SAM.
The impact of backfiller composition should be taken into account. (Adapted from [36], with
permission from Elsevier)
52
J.-A. Preuß et al.
aptamer-based cancer treatment by oncolytic virus.
2.2.1 Cancer Cells
The state-of-the-art diagnostics of cancer types that are based on imaging methods
are limited since they mainly analyze morphology. However, morphology-based
classification does not allow for early-stage-diagnosis as morphologic changes do
not occur right away. As an alternative, impedimetric aptasensors are an opportunity
of rapid and label-free detection of cancer cells themselves. For instance, CT26
(colorectal cancer) were detected by an impedimetric aptasensor as a rapid and labelfree approach [39]. The proposed sensor enabled LoD of 2 cells/ml and a linear range
of 1 Â 10
5 –6 Â 10
6 cells/ml.
Diagnosis of hepatocellular carcinoma (HCC) goes along with poor prognosis for
survival the next 5 years due to the lack of effective treatment strategies. So far, the
Fig. 3 Gold electrodes are often functionalized by well-known gold sulfur chemistry. In order to
achieve a self-assembled monolayer (SAM) and to reduce non-specific binding, the application of
short backfillers is a common strategy besides co-immobilization or post-functionalization of SAM.
The impact of backfiller composition should be taken into account. (Adapted from [36], with
permission from Elsevier)
52
J.-A. Preuß et al.