30. Masferrer J, Albertini R, Croxatto HR, García P, Pinto I (1985) Isolation and characterization
of rat plasma glandular kallikrein. Biochem Pharmacol 34:51–56
31. Dean PD, Watson DH (1979) Protein purification using immobilised triazine dyes.
J Chromatogr 165:301–319
32. Stellwagen E (1990) Chromatography on immobilized reactive dyes. Methods Enzymol
182:343–357
33. Hongo S, Sato T (1981) Purification of rat liver asparagine synthetase by affinity chromatography on reactive blue 2-agarose. Anal Biochem 114:163–166
34. Birkenmeier G, Usbeck E, Saro L, Kopperschläger G (1983) Triazine dye binding of human
alpha-fetoprotein and albumin. J Chromatogr 265:27–35
35. Naumann M, Reuter R, Metz P, Kopperschläger G (1989) Affinity chromatography of bovine
heart lactate dehydrogenase using dye ligands linked directly or spacer-mediated to bead
cellulose. J Chromatogr 466:319–329
36. Lowe CR, Burton SJ, Burton NP, Alderton WK, Pitts JM, Thomas JA (1992) Designer dyes:
‘biomimetic’ ligands for the purification of pharmaceutical proteins by affinity chromatography. Trends Biotechnol 10:442–448
37. El Khoury G, Wang Y, Wang D, Jacob SI, Lowe CR (2013) Design, synthesis, and assessment
of a de novo affinity adsorbent for the purification of recombinant human erythropoietin.
Biotechnol Bioeng 110:3063–3069
38. Teng SF, Sproule K, Husain A, Lowe CR (2000) Affinity chromatography on immobilized
“biomimetic” ligands. Synthesis, immobilization and chromatographic assessment of an
immunoglobulin G-binding ligand. J Chromatogr B 740:1–15
39. Roque AC, Lowe CR (2006) Advances and applications of de novo designed affinity ligands
in proteomics. Biotechnol Adv 24:17–26
40. El Khoury G, Rowe LA, Lowe CR (2012) Biomimetic affinity ligands for immunoglobulins
based on the multicomponent Ugi reaction. Methods Mol Biol 800:57–74
41. Furka A, Sebestyen F, Asgedom M, Dibo G (1991) General method for rapid synthesis of
multicomponent peptide mixtures. Int J Pept Protein Res 37:487–493
42. Menegatti S, Naik AD, Gurgel PV, Carbonell RG (2012) Alkaline-stable peptide ligand
affinity adsorbents for the purification of biomolecules. J Chromatogr A 1245:55–64
43. Kish WS, Roach MK, Sachi H, Naik AD, Menegatti S, Carbonell RG (2018) Purification of
human erythropoietin by affinity chromatography using cyclic peptide ligands. J Chromatogr
B 1085:1–12
44. Fang Y-M, Lin D-Q, Yao S-J (2018) Review on biomimetic affinity chromatography with
short peptide ligands and its application to protein purification. J Chromatogr A 1571:1–15
45. Nord K, Gunneriusson E, Ringdahl J, Ståhl S, Uhlén M, Nygren PA (1997) Binding proteins
selected from combinatorial libraries of an alpha-helical bacterial receptor domain. Nat
Biotechnol 15:772–777
46. Wållberg H, Löfdahl PK, Tschapalda K, Uhlén M, Tolmachev V, Nygren PK, Ståhl S (2011)
Affinity recovery of eight HER2-binding affibody variants using an anti-idiotypic affibody
molecule as capture ligand. Protein Expr Purif 76:127–135
47. Hamers-Casterman C, Atarhouch T, Muyldermans S, Robinson G, Hamers C, Songa EB,
Bendahman N, Hamers R (1993) Naturally occurring antibodies devoid of light chains. Nature
363:446–448
48. Georgiou G, Stathopoulos C, Daugherty PS, Nayak AR, Iverson BL, Curtiss 3rd R (1997)
Display of heterologous proteins on the surface of microorganisms: from the screening of
combinatorial libraries to live recombinant vaccines. Nat Biotechnol 15:29–34
49. Lam KS, Lebl M, Krchnák V (1997) The “one-bead-one-compound” combinatorial library
method. Chem Rev 97:411–448
50. Wang Y, Li T (2002) Identification of affinity ligands for protein purification from synthetic
chemical combinatorial libraries. Biotechnol Prog 18:524–529
51. Lathrop JT, Fijalkowska I, Hammond D (2007) The Bead blot: a method for identifying
ligand-protein and protein-protein interactions using combinatorial libraries of peptide ligands.
Anal Biochem 361:65–76
132
G. Perret and E. Boschetti
of rat plasma glandular kallikrein. Biochem Pharmacol 34:51–56
31. Dean PD, Watson DH (1979) Protein purification using immobilised triazine dyes.
J Chromatogr 165:301–319
32. Stellwagen E (1990) Chromatography on immobilized reactive dyes. Methods Enzymol
182:343–357
33. Hongo S, Sato T (1981) Purification of rat liver asparagine synthetase by affinity chromatography on reactive blue 2-agarose. Anal Biochem 114:163–166
34. Birkenmeier G, Usbeck E, Saro L, Kopperschläger G (1983) Triazine dye binding of human
alpha-fetoprotein and albumin. J Chromatogr 265:27–35
35. Naumann M, Reuter R, Metz P, Kopperschläger G (1989) Affinity chromatography of bovine
heart lactate dehydrogenase using dye ligands linked directly or spacer-mediated to bead
cellulose. J Chromatogr 466:319–329
36. Lowe CR, Burton SJ, Burton NP, Alderton WK, Pitts JM, Thomas JA (1992) Designer dyes:
‘biomimetic’ ligands for the purification of pharmaceutical proteins by affinity chromatography. Trends Biotechnol 10:442–448
37. El Khoury G, Wang Y, Wang D, Jacob SI, Lowe CR (2013) Design, synthesis, and assessment
of a de novo affinity adsorbent for the purification of recombinant human erythropoietin.
Biotechnol Bioeng 110:3063–3069
38. Teng SF, Sproule K, Husain A, Lowe CR (2000) Affinity chromatography on immobilized
“biomimetic” ligands. Synthesis, immobilization and chromatographic assessment of an
immunoglobulin G-binding ligand. J Chromatogr B 740:1–15
39. Roque AC, Lowe CR (2006) Advances and applications of de novo designed affinity ligands
in proteomics. Biotechnol Adv 24:17–26
40. El Khoury G, Rowe LA, Lowe CR (2012) Biomimetic affinity ligands for immunoglobulins
based on the multicomponent Ugi reaction. Methods Mol Biol 800:57–74
41. Furka A, Sebestyen F, Asgedom M, Dibo G (1991) General method for rapid synthesis of
multicomponent peptide mixtures. Int J Pept Protein Res 37:487–493
42. Menegatti S, Naik AD, Gurgel PV, Carbonell RG (2012) Alkaline-stable peptide ligand
affinity adsorbents for the purification of biomolecules. J Chromatogr A 1245:55–64
43. Kish WS, Roach MK, Sachi H, Naik AD, Menegatti S, Carbonell RG (2018) Purification of
human erythropoietin by affinity chromatography using cyclic peptide ligands. J Chromatogr
B 1085:1–12
44. Fang Y-M, Lin D-Q, Yao S-J (2018) Review on biomimetic affinity chromatography with
short peptide ligands and its application to protein purification. J Chromatogr A 1571:1–15
45. Nord K, Gunneriusson E, Ringdahl J, Ståhl S, Uhlén M, Nygren PA (1997) Binding proteins
selected from combinatorial libraries of an alpha-helical bacterial receptor domain. Nat
Biotechnol 15:772–777
46. Wållberg H, Löfdahl PK, Tschapalda K, Uhlén M, Tolmachev V, Nygren PK, Ståhl S (2011)
Affinity recovery of eight HER2-binding affibody variants using an anti-idiotypic affibody
molecule as capture ligand. Protein Expr Purif 76:127–135
47. Hamers-Casterman C, Atarhouch T, Muyldermans S, Robinson G, Hamers C, Songa EB,
Bendahman N, Hamers R (1993) Naturally occurring antibodies devoid of light chains. Nature
363:446–448
48. Georgiou G, Stathopoulos C, Daugherty PS, Nayak AR, Iverson BL, Curtiss 3rd R (1997)
Display of heterologous proteins on the surface of microorganisms: from the screening of
combinatorial libraries to live recombinant vaccines. Nat Biotechnol 15:29–34
49. Lam KS, Lebl M, Krchnák V (1997) The “one-bead-one-compound” combinatorial library
method. Chem Rev 97:411–448
50. Wang Y, Li T (2002) Identification of affinity ligands for protein purification from synthetic
chemical combinatorial libraries. Biotechnol Prog 18:524–529
51. Lathrop JT, Fijalkowska I, Hammond D (2007) The Bead blot: a method for identifying
ligand-protein and protein-protein interactions using combinatorial libraries of peptide ligands.
Anal Biochem 361:65–76
132
G. Perret and E. Boschetti