289
capacity of colonization nor inhibited the formation of the different characteristic
structures of AMF, such as arbuscules, vesicles, and hyphae. Furthermore, in unfertilized plants, AMF inocula promoted growth. The increased leaf production in
inoculated plants suggests that mycorrhizae stimulate ontogeny and delay leaf
senescence. In addition, increasing the fertilizer to 100% improved growth in the
treatment with the PL inoculum, and on the contrary, the MIX and CB inocula had
less effect than fertilization of 100% in most of the variables of increase. On the
other hand, the resprouting capacity of P. alba after drought stress conditions varied
with different levels of fertilization and with different types of inoculum. In this
context, the uninoculated and unfertilized treatment exhibited the lowest resprouting rate of all the treatments. On the contrary, the effect of the treatment (AMF × fertilization) was significant (p = 0.0198) increasing the resprouting capacity.
Subsequent comparisons indicate that the proportion of resprouted plants in PL_0%
and PL_ 25% treatments were significantly higher (Fig. 10.9). These results demonstrated that the AMF inoculum isolated from the semiarid regions of the study area,
such as PL, may have the potential to mitigate drought stress in P. alba seedlings
compared to the inoculum isolated from wet areas (Salto et al. 2019b).
0.8
A
A
B
B
B
B
B
B
B
B
B
B
0.7
0.6
0.5
0.4
0.3
0.2
0.1
AMF Inoculum x Fertilization
Proportion of resprouted seedlings
0
P L _ 2 5
P L _ 1 0
W L _ 1 0 0
C B _ 1 0 0
M I X _ 2 5
M I X _ 1 0 0
P L _ 1 0 0
C B _ 0
M I X _ 0
W I _ 2 5
C B _ 2 5
W I _ 0
Fig. 10.9 Effect of different fertilization and AMF inoculation regimes on P. alba seedlings submitted to drought stress. Resprouting capacity is expressed as the proportion of resprouted plants
after 15 days of drought stress. Mean values and their standard errors. Different letters indicate
significant differences (p ≤ 0.05) according to DGC’s test (Salto et al. 2019b). PL Padre Lozano
inoculum, CB Colonia Benitez inoculum, MIX mixture of both inocula, WI without inoculum, 100
fertilization without dilution, 25 fertilization diluted to 25%, 0 without fertilization
10 Genetic Breeding of Prosopis Species from the “Great American Chaco”
capacity of colonization nor inhibited the formation of the different characteristic
structures of AMF, such as arbuscules, vesicles, and hyphae. Furthermore, in unfertilized plants, AMF inocula promoted growth. The increased leaf production in
inoculated plants suggests that mycorrhizae stimulate ontogeny and delay leaf
senescence. In addition, increasing the fertilizer to 100% improved growth in the
treatment with the PL inoculum, and on the contrary, the MIX and CB inocula had
less effect than fertilization of 100% in most of the variables of increase. On the
other hand, the resprouting capacity of P. alba after drought stress conditions varied
with different levels of fertilization and with different types of inoculum. In this
context, the uninoculated and unfertilized treatment exhibited the lowest resprouting rate of all the treatments. On the contrary, the effect of the treatment (AMF × fertilization) was significant (p = 0.0198) increasing the resprouting capacity.
Subsequent comparisons indicate that the proportion of resprouted plants in PL_0%
and PL_ 25% treatments were significantly higher (Fig. 10.9). These results demonstrated that the AMF inoculum isolated from the semiarid regions of the study area,
such as PL, may have the potential to mitigate drought stress in P. alba seedlings
compared to the inoculum isolated from wet areas (Salto et al. 2019b).
0.8
A
A
B
B
B
B
B
B
B
B
B
B
0.7
0.6
0.5
0.4
0.3
0.2
0.1
AMF Inoculum x Fertilization
Proportion of resprouted seedlings
0
P L _ 2 5
P L _ 1 0
W L _ 1 0 0
C B _ 1 0 0
M I X _ 2 5
M I X _ 1 0 0
P L _ 1 0 0
C B _ 0
M I X _ 0
W I _ 2 5
C B _ 2 5
W I _ 0
Fig. 10.9 Effect of different fertilization and AMF inoculation regimes on P. alba seedlings submitted to drought stress. Resprouting capacity is expressed as the proportion of resprouted plants
after 15 days of drought stress. Mean values and their standard errors. Different letters indicate
significant differences (p ≤ 0.05) according to DGC’s test (Salto et al. 2019b). PL Padre Lozano
inoculum, CB Colonia Benitez inoculum, MIX mixture of both inocula, WI without inoculum, 100
fertilization without dilution, 25 fertilization diluted to 25%, 0 without fertilization
10 Genetic Breeding of Prosopis Species from the “Great American Chaco”