detects single bases as they are incorporated into DNA template strands. As all four
reversible terminator-bound dNTPs are present as single, separate molecules during each
sequencing cycle, natural competition minimizes incorporation bias and greatly reduces
raw error rates. The result is highly accurate base-by-base sequencing that virtually
eliminates sequence context-specific errors, even within repetitive sequence regions and
homopolymers (see Fig. 4.1c). During data analysis and alignment, the newly identified
Fig. 4.1 Next Generation Sequencing Chemistry Overview—Illumina NGS includes four steps: (a)
library preparation, (b) cluster generation, (c) sequencing, and (d) alignment and data analysis.
(source: www.illumina.com)
50
M. Eisele and M. Kappelmann-Fenzl
reversible terminator-bound dNTPs are present as single, separate molecules during each
sequencing cycle, natural competition minimizes incorporation bias and greatly reduces
raw error rates. The result is highly accurate base-by-base sequencing that virtually
eliminates sequence context-specific errors, even within repetitive sequence regions and
homopolymers (see Fig. 4.1c). During data analysis and alignment, the newly identified
Fig. 4.1 Next Generation Sequencing Chemistry Overview—Illumina NGS includes four steps: (a)
library preparation, (b) cluster generation, (c) sequencing, and (d) alignment and data analysis.
(source: www.illumina.com)
50
M. Eisele and M. Kappelmann-Fenzl