Table 1
Major NGS platforms and their general properties
Platform
Company
Instrument
Reads per run
Average
read
length
[bp]
Read
type
Error
RATE
Data
generated
per run
(Gb)
Template
(single
molecule
or clonal)
Sequencing
scheme
Cyclic or
continous Vizualization
Sequencing
principle
Sanger
sequencing
ThermoFisher/
Roche
ABI 3500/
3730
//
Up to
1 kb
Primer
defined
//
0,0003
Clonal
//
//
Optical,
electrophoresis
//
MiniSeq
25 Mio.
1
 75
to
2
 150
Short
1/1000 1.7–7.5
Clonal
Surface or
microwells
Cyclic
Optical
Terminated
synthesis
MiSeq
25 Mio.
1
 36
to
2
 300
Short
1/1000 0.3–15
Clonal
Surface or
microwells
Cyclic
Optical
Terminated
synthesis
NextSeq
2000
400 Mio.
1
 75
to
2
 150
Short
1/1000 10–120
Clonal
Surface or
microwells
Cyclic
Optical
Terminated
synthesis
HiSeq 4000 5 bio.
1
 50
to
2
 250
Short
1/1000 10–1000
Clonal
Surface or
microwells
Cyclic
Optical
Terminated
synthesis
NovaSeq
5000/6000
20 bio.
2
 50
to
2
 150
Short
1/1000 2000–6000 Clonal
Surface or
microwells
Cyclic
Optical
Terminated
synthesis
IonTorrent ThermoFischer PGM
5.5 Mio.
Up to
400
Short
~2.5% 0.08–2
Clonal
Beads in
wells
Cyclic
Electrical
Unterminated
synthesis
S5
2–130 Mio.
Up to
400
Short
~1%
0.6–15
Clonal
Beads in
wells
Cyclic
Electrical
Unterminated
synthesis
Proton
40–80 Mio.
Up to
200
Short
~1%
10–15
Clonal
Beads in
wells
Cyclic
Electrical
Unterminated
synthesis
Pacific
Bioscience
Pacific
Bioscience
Illumina
PacBio RSII 50 T
Up to
60 kb
Long
1/100
10
Single
molecule
Microwells Continous Optical
Unterminated
synthesis
Sequel
500 T
Up to
60 kb
Long
1/100
15
Single
molecule
Microwells Continous Optical
Unterminated
synthesis
Sequel II
4 Mio.
Up to
60 kb
Long
1/100
100
Single
molecule
Microwells Continous Optical
Unterminated
synthesis
(continued)
Appendix
209