Next Generation Sequencing (NGS): What Can
Be Sequenced?
1
Anja Bosserhoff and Melanie Kappelmann-Fenzl
Contents
1.1 Introduction . . . .... ..... .... ..... .... ..... .... ..... ..... .... ..... .... ..... .... ..... .... ..... .... . 2
1.2 Biological Sequences . . . . . . .. . . . . .. . . . . .. . . . .. . . . . .. . . . . .. . . . . .. . . . . .. . . . . .. . . . . .. . . . . .. . . . . .. . 2
1.2.1 DNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
1.2.2 Protein . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
1.2.3 Other Important Features of the Genome . . . . .. . . . .. . . . . .. . . . .. . . . . .. . . . . .. . . . .. . . . . .. 6
References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
What You Will Learn in This Chapter
The content of the following chapter briefly describes the basics of biological genetic
information and their function in living organisms. You will gain principle knowledge on DNA and RNA and understand the differences of analyzing these by
sequencing templates. Next generation sequencing technologies can be used to
(continued)
A. Bosserhoff
Institute of Biochemistry (Emil-Fischer Center), Friedrich–Alexander University Erlangen–
Nürnberg, Erlangen, Germany
M. Kappelmann-Fenzl (*)
Institute of Biochemistry (Emil-Fischer Center), Friedrich–Alexander University Erlangen–
Nürnberg, Erlangen, Germany
Deggendorf Institute of Technology, Deggendorf, Germany
e-mail: melanie.kappelmann-fenzl@th-deg.de
# Springer Nature Switzerland AG 2021
M. Kappelmann-Fenzl (ed.), Next Generation Sequencing and Data Analysis, Learning
Materials in Biosciences, https://doi.org/10.1007/978-3-030-62490-3_1
1
Be Sequenced?
1
Anja Bosserhoff and Melanie Kappelmann-Fenzl
Contents
1.1 Introduction . . . .... ..... .... ..... .... ..... .... ..... ..... .... ..... .... ..... .... ..... .... ..... .... . 2
1.2 Biological Sequences . . . . . . .. . . . . .. . . . . .. . . . .. . . . . .. . . . . .. . . . . .. . . . . .. . . . . .. . . . . .. . . . . .. . . . . .. . 2
1.2.1 DNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
1.2.2 Protein . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
1.2.3 Other Important Features of the Genome . . . . .. . . . .. . . . . .. . . . .. . . . . .. . . . . .. . . . .. . . . . .. 6
References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
What You Will Learn in This Chapter
The content of the following chapter briefly describes the basics of biological genetic
information and their function in living organisms. You will gain principle knowledge on DNA and RNA and understand the differences of analyzing these by
sequencing templates. Next generation sequencing technologies can be used to
(continued)
A. Bosserhoff
Institute of Biochemistry (Emil-Fischer Center), Friedrich–Alexander University Erlangen–
Nürnberg, Erlangen, Germany
M. Kappelmann-Fenzl (*)
Institute of Biochemistry (Emil-Fischer Center), Friedrich–Alexander University Erlangen–
Nürnberg, Erlangen, Germany
Deggendorf Institute of Technology, Deggendorf, Germany
e-mail: melanie.kappelmann-fenzl@th-deg.de
# Springer Nature Switzerland AG 2021
M. Kappelmann-Fenzl (ed.), Next Generation Sequencing and Data Analysis, Learning
Materials in Biosciences, https://doi.org/10.1007/978-3-030-62490-3_1
1