100
90
80
70
60
50
40
30
20
10
0
1
2
3
4
5
6
7
8
9
Sequence Duplication Level
Percent of seqs remaining if deduplicated 99.31%
% Deduplicated sequences
% Total sequences
>10
>50 >100 >500
>1k
>5k
>10k
Fig. 7.15 DNA library sequence duplication. (source: https://rtsf.natsci.msu.edu)
Percent of seqs remaining if deduplicated 30.04%
100
90
80
70
60
50
40
30
20
10
0
Sequence Duplication Level
1
2
3
4
5
6
7
8
9
>10
>50 >100 >500 >1k
>5k >10k
% Deduplicated sequences
% Total sequences
Fig. 7.16 RNA library sequence duplication. (source: https://rtsf.natsci.msu.edu)
7 NGS Data
101