16. Ladbury JE, Klebe G, Freire E (2010) Adding
calorimetric data to decision making in lead
discovery: a hot tip. Nat Rev Drug Discov
9:23–27
17. Olsson TSG, Williams MA, Pitt WR, Ladbury
JE (2008) The thermodynamics of protein–ligand interaction and solvation: insights for
ligand design. J Mol Biol 384:1002–1017
18. Karlsson R, Michaelsson A, Mattsson L (1991)
Kinetic analysis of monoclonal antibodyantigen interactions with a new biosensor
based analytical system. J Immunol Methods
145:229–240
19. Schasfoort RBM (2017) Handbook of surface
plasmon resonance. The Royal Society of
Chemistry, London
20. Kemmer G, Keller S (2010) Nonlinear leastsquares data fitting in Excel spreadsheets. Nat
Protoc 5:267–281
21. Keller S, Vargas C, Zhao H, Piszczek G, Brautigam CA, Schuck P (2012) High-precision
isothermal titration calorimetry with automated peak-shape analysis. Anal Chem
84:5066–5073
22. Scheuermann TH, Brautigam CA (2015)
High-precision, automated integration of multiple isothermal titration calorimetric thermograms: new features of NITPIC. Methods
76:87–98
23. Zhao H, Piszczek G, Schuck P (2015) SEDPHAT--a platform for global ITC analysis and
global multi-method analysis of molecular
interactions. Methods 76:137–148
24. Sophianopoulos AJ, Van Holde KE (1964)
Physical studies of muramidase (lysozyme)
II. pH-dependent dimerization. J Biol Chem
239:2516–2524
25. Pace CN, Vajdos F, Fee L, Grimsley G, Gray T
(1995) How to measure and predict the molar
absorption coefficient of a protein. Protein Sci
4:2411–2423
26. Fedorov O, Niesen FH, Knapp S (2012)
Kinase inhibitor selectivity profiling using differential scanning fluorimetry. In: Kuster B
(ed) Kinase inhibitors: methods and protocols.
Humana Press, Totowa, NJ
27. Scheuermann TH, Padrick SB, Gardner KH,
Brautigam CA (2016) On the acquisition and
analysis of microscale thermophoresis data.
Anal Biochem 496:79–93
28. Brown PH, Balbo A, Schuck P (2008) Characterizing protein-protein interactions by sedimentation
velocity
analytical
ultracentrifugation. Curr Protoc Immunol
81:18.15.1–18.15.39
29. Pope AJ, Haupts UM, Moore KJ (1999)
Homogeneous fluorescence readouts for miniaturized high-throughput screening: theory
and practice. Drug Discov Today 4:350–362
30. Mizoue LS, Tellinghuisen J (2004) The role of
backlash in the “first injection anomaly” in isothermal titration calorimetry. Anal Biochem
326:125–127
Interactions by Multiple Methods
79
calorimetric data to decision making in lead
discovery: a hot tip. Nat Rev Drug Discov
9:23–27
17. Olsson TSG, Williams MA, Pitt WR, Ladbury
JE (2008) The thermodynamics of protein–ligand interaction and solvation: insights for
ligand design. J Mol Biol 384:1002–1017
18. Karlsson R, Michaelsson A, Mattsson L (1991)
Kinetic analysis of monoclonal antibodyantigen interactions with a new biosensor
based analytical system. J Immunol Methods
145:229–240
19. Schasfoort RBM (2017) Handbook of surface
plasmon resonance. The Royal Society of
Chemistry, London
20. Kemmer G, Keller S (2010) Nonlinear leastsquares data fitting in Excel spreadsheets. Nat
Protoc 5:267–281
21. Keller S, Vargas C, Zhao H, Piszczek G, Brautigam CA, Schuck P (2012) High-precision
isothermal titration calorimetry with automated peak-shape analysis. Anal Chem
84:5066–5073
22. Scheuermann TH, Brautigam CA (2015)
High-precision, automated integration of multiple isothermal titration calorimetric thermograms: new features of NITPIC. Methods
76:87–98
23. Zhao H, Piszczek G, Schuck P (2015) SEDPHAT--a platform for global ITC analysis and
global multi-method analysis of molecular
interactions. Methods 76:137–148
24. Sophianopoulos AJ, Van Holde KE (1964)
Physical studies of muramidase (lysozyme)
II. pH-dependent dimerization. J Biol Chem
239:2516–2524
25. Pace CN, Vajdos F, Fee L, Grimsley G, Gray T
(1995) How to measure and predict the molar
absorption coefficient of a protein. Protein Sci
4:2411–2423
26. Fedorov O, Niesen FH, Knapp S (2012)
Kinase inhibitor selectivity profiling using differential scanning fluorimetry. In: Kuster B
(ed) Kinase inhibitors: methods and protocols.
Humana Press, Totowa, NJ
27. Scheuermann TH, Padrick SB, Gardner KH,
Brautigam CA (2016) On the acquisition and
analysis of microscale thermophoresis data.
Anal Biochem 496:79–93
28. Brown PH, Balbo A, Schuck P (2008) Characterizing protein-protein interactions by sedimentation
velocity
analytical
ultracentrifugation. Curr Protoc Immunol
81:18.15.1–18.15.39
29. Pope AJ, Haupts UM, Moore KJ (1999)
Homogeneous fluorescence readouts for miniaturized high-throughput screening: theory
and practice. Drug Discov Today 4:350–362
30. Mizoue LS, Tellinghuisen J (2004) The role of
backlash in the “first injection anomaly” in isothermal titration calorimetry. Anal Biochem
326:125–127
Interactions by Multiple Methods
79