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1150111003
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background silver diamine and silver nitrate
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https://doi.org/10.1002/elps.1150130190
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proteomics protocol handbook. Humana
Press, Totowa, NJ, pp 215–222
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light-induced reactions of tryptophan with
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Photochem
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0031-8655(2002)0750362tlirot2.0.co2
53. Voytas D, Ke N (2001) Detection and quantitation of radiolabeled proteins in gels and blots.
Curr Protoc Cell Biol Chapter 6:Unit 6.3.
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Bertrand Raynal et al.
determination by UV absorption. In: Walker
JM (ed) The protein protocols handbook.
Humana Press, Totowa, NJ, pp 3–6
43. Kuipers BJH, Gruppen H (2007) Prediction of
molar extinction coefficients of proteins and
peptides using UV absorption of the constituent amino acids at 214 nm to enable quantitative reverse phase high-performance liquid
chromatography-mass spectrometry analysis. J
Agric Food Chem 55:5445–5451. https://doi.
org/10.1021/jf070337l
44. Sch€ agger H, von Jagow G (1987) Tricinesodium dodecyl sulfate-polyacrylamide gel
electrophoresis for the separation of proteins
in the range from 1 to 100 kDa. Anal Biochem
166:368–379.
https://doi.org/10.1016/
0003-2697(87)90587-2
45. Lo M-C, Aulabaugh A, Jin G, Cowling R,
Bard J, Malamas M, Ellestad G (2004) Evaluation of fluorescence-based thermal shift assays
for hit identification in drug discovery. Anal
Biochem 332:153–159. https://doi.org/10.
1016/j.ab.2004.04.031
46. Macheroux P (1999) UV-visible spectroscopy
as a tool to study flavoproteins. In: Chapman
SK, Reid GA (eds) Flavoprotein protocols.
Humana Press, Totowa, NJ, pp 1–7
47. Micsonai A, Wien F, Kernya L, Lee Y-H,
Goto Y, Re ´fre ´giers M, Kardos J (2015) Accurate secondary structure prediction and fold
recognition for circular dichroism spectroscopy. Proc Natl Acad Sci U S A 112:
E3095–E3103.
https://doi.org/10.1073/
pnas.1500851112
48. Micsonai A, Wien F, Bulya ´ki E, Kun J,
Moussong E, Lee Y-H, Goto Y, Re ´fre ´giers M,
Kardos J (2018) BeStSel: a web server for accurate protein secondary structure prediction and
fold recognition from the circular dichroism
spectra. Nucleic Acids Res 46:W315–W322.
https://doi.org/10.1093/nar/gky497
49. Rabilloud T (1990) Mechanisms of protein
silver staining in polyacrylamide gels: a
10-year
synthesis.
Electrophoresis
11:785–794. https://doi.org/10.1002/elps.
1150111003
50. Rabilloud T (1992) A comparison between low
background silver diamine and silver nitrate
protein stains. Electrophoresis 13:429–439.
https://doi.org/10.1002/elps.1150130190
51. Fernandez-Patron C (2005) Zn
2+ reverse
staining technique. In: Walker JM (ed) The
proteomics protocol handbook. Humana
Press, Totowa, NJ, pp 215–222
52. Edwards RA, Jickling G, Turner RJ (2002) The
light-induced reactions of tryptophan with
halocompounds.
Photochem
Photobiol
75:362–368.
https://doi.org/10.1562/
0031-8655(2002)0750362tlirot2.0.co2
53. Voytas D, Ke N (2001) Detection and quantitation of radiolabeled proteins in gels and blots.
Curr Protoc Cell Biol Chapter 6:Unit 6.3.
https://doi.org/10.1002/0471143030.
cb0603s10
46
Bertrand Raynal et al.