Editors
Tina Daviter
The Institute of Cancer Research
London, UK
Christopher M. Johnson
MRC Laboratory of Molecular Biology
Cambridge, UK
Stephen H. McLaughlin
MRC Laboratory of Molecular Biology
Cambridge, UK
Mark A. Williams
ISMB BiophysX Centre, Birkbeck
University of London
London, UK
ISSN 1064-3745
ISSN 1940-6029 (electronic)
Methods in Molecular Biology
ISBN 978-1-0716-1196-8
ISBN 978-1-0716-1197-5 (eBook)
https://doi.org/10.1007/978-1-0716-1197-5
© Springer Science+Business Media, LLC, part of Springer Nature 2021
This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is
concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction
on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation,
computer software, or by similar or dissimilar methodology now known or hereafter developed.
The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply,
even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations
and therefore free for general use.
The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to
be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty,
expressed or implied, with respect to the material contained herein or for any errors or omissions that may have been
made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Front cover: Interaction of initiator tRNA (teal) and initiation factors IF1 (purple) and IF3 (orange) with the small
ribosomal subunit (30S) from structural work on the pre-initiation complexes determined in the lab of Venki
Ramakrishnan. Copyright Visual Aids, MRC Laboratory of Molecular Biology, Cambridge.
This Humana imprint is published by the registered company Springer Science+Business Media, LLC, part of Springer
Nature.
The registered company address is: 1 New York Plaza, New York, NY 10004, U.S.A.
Tina Daviter
The Institute of Cancer Research
London, UK
Christopher M. Johnson
MRC Laboratory of Molecular Biology
Cambridge, UK
Stephen H. McLaughlin
MRC Laboratory of Molecular Biology
Cambridge, UK
Mark A. Williams
ISMB BiophysX Centre, Birkbeck
University of London
London, UK
ISSN 1064-3745
ISSN 1940-6029 (electronic)
Methods in Molecular Biology
ISBN 978-1-0716-1196-8
ISBN 978-1-0716-1197-5 (eBook)
https://doi.org/10.1007/978-1-0716-1197-5
© Springer Science+Business Media, LLC, part of Springer Nature 2021
This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is
concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction
on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation,
computer software, or by similar or dissimilar methodology now known or hereafter developed.
The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply,
even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations
and therefore free for general use.
The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to
be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty,
expressed or implied, with respect to the material contained herein or for any errors or omissions that may have been
made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Front cover: Interaction of initiator tRNA (teal) and initiation factors IF1 (purple) and IF3 (orange) with the small
ribosomal subunit (30S) from structural work on the pre-initiation complexes determined in the lab of Venki
Ramakrishnan. Copyright Visual Aids, MRC Laboratory of Molecular Biology, Cambridge.
This Humana imprint is published by the registered company Springer Science+Business Media, LLC, part of Springer
Nature.
The registered company address is: 1 New York Plaza, New York, NY 10004, U.S.A.