10. Butterer A, Pernstich C, Smith RM, Sobott F,
Szczelkun MD, Toth J (2014) Type III restriction endonucleases are heterotrimeric: comprising one helicase-nuclease subunit and a
dimeric methyltransferase that binds only one
specific DNA. Nucleic Acids Res 42
(8):5139–5150. https://doi.org/10.1093/
nar/gku122
11. Deng X, Prakash A, Dhar K, Baia GS, Kolar C,
Oakley GG, Borgstahl GE (2009) Human replication protein A-Rad52-single-stranded
DNA complex: stoichiometry and evidence
for strand transfer regulation by phosphorylation.
Biochemistry
48(28):6633–6643.
https://doi.org/10.1021/bi900564k
12. Porterfield JZ, Zlotnick A (2010) A simple and
general method for determining the protein
and nucleic acid content of viruses by UV
absorbance.
Virology
407(2):281–288.
https://doi.org/10.1016/j.virol.2010.08.
015
13. Aceytuno RD, Piett CG, Havali-Shahriari Z,
Edwards RA, Rey M, Ye R, Javed F, Fang S,
Mani R, Weinfeld M, Hammel M, Tainer JA,
Schriemer DC, Lees-Miller SP, Glover JNM
(2017) Structural and functional characterization of the PNKP-XRCC4-LigIV DNA repair
complex.
Nucleic
Acids
Res
45
(10):6238–6251. https://doi.org/10.1093/
nar/gkx275
14. Hsiao HH, Nath A, Lin CY, Folta-Stogniew
EJ, Rhoades E, Braddock DT (2010) Quantitative characterization of the interactions
among c-myc transcriptional regulators
FUSE, FBP, and FIR. Biochemistry 49
(22):4620–4634. https://doi.org/10.1021/
bi9021445
15. French RL, Gupta N, Copeland PR, Simonovic
M (2014) Structural asymmetry of the terminal
catalytic complex in selenocysteine synthesis. J
Biol Chem 289(42):28783–28794. https://
doi.org/10.1074/jbc.M114.597955
16. Aik WS, Lin MH, Tan D, Tripathy A, Marzluff
WF, Dominski Z, Chou CY, Tong L (2017)
The N-terminal domains of FLASH and
Lsm11 form a 2:1 heterotrimer for histone
pre-mRNA 3
0 -end processing. PLoS One 12
(10):e0186034. https://doi.org/10.1371/
journal.pone.0186034
17. Wen J, Arakawa T, Wypych J, Langley KE,
Schwartz MG, Philo JS (1997) Chromatographic determination of extinction coefficients of non-glycosylated proteins using
refractive index (RI) and UV absorbance
(UV) detectors: applications for studying protein interactions by size exclusion chromatography with light-scattering, UV, and RI
detectors. In: Marshak DR (ed) Techniques in
protein chemistry, vol 8. Academic, New York,
pp
113–119.
https://doi.org/10.1016/
S1080-8914(97)80014-2
18. Stevens FJ (1989) Analysis of protein-protein
interaction by simulation of small-zone size
exclusion chromatography. Stochastic formulation of kinetic rate contributions to observed
high-performance liquid chromatography elution
characteristics.
Biophys
J
55
(6):1155–1167. https://doi.org/10.1016/
s0006-3495(89)82912-1
19. Giddings J (1993) Field-flow fractionation:
analysis of macromolecular, colloidal, and particulate
materials.
Science
260
(5113):1456–1465.
https://doi.org/10.
1126/science.8502990
20. Ashby J, Schachermeyer S, Duan Y, Jimenez
LA, Zhong W (2014) Probing and quantifying
DNA–protein interactions with asymmetrical
flow field-flow fractionation. J Chromatogr A
1358:217–224. https://doi.org/10.1016/j.
chroma.2014.07.002
21. Kendrick BS, Kerwin BA, Chang BS, Philo JS
(2001) Online size-exclusion high-performance liquid chromatography light scattering
and differential refractometry methods to
determine degree of polymer conjugation to
proteins and protein-protein or protein-ligand
association states. Anal Biochem 299
(2):136–146. https://doi.org/10.1006/abio.
2001.5411
22. Zhao H, Brown PH, Schuck P (2011) On the
distribution of protein refractive index increments. Biophys J 100(9):2309–2317. https://
doi.org/10.1016/j.bpj.2011.03.004
23. Nishino T, Rago F, Hori T, Tomii K, Cheeseman IM, Fukagawa T (2013) CENP-T provides a structural platform for outer
kinetochore
assembly.
EMBO
J
32
(3):424–436.
https://doi.org/10.1038/
emboj.2012.348
SEC Coupled with Light Scattering
395
Szczelkun MD, Toth J (2014) Type III restriction endonucleases are heterotrimeric: comprising one helicase-nuclease subunit and a
dimeric methyltransferase that binds only one
specific DNA. Nucleic Acids Res 42
(8):5139–5150. https://doi.org/10.1093/
nar/gku122
11. Deng X, Prakash A, Dhar K, Baia GS, Kolar C,
Oakley GG, Borgstahl GE (2009) Human replication protein A-Rad52-single-stranded
DNA complex: stoichiometry and evidence
for strand transfer regulation by phosphorylation.
Biochemistry
48(28):6633–6643.
https://doi.org/10.1021/bi900564k
12. Porterfield JZ, Zlotnick A (2010) A simple and
general method for determining the protein
and nucleic acid content of viruses by UV
absorbance.
Virology
407(2):281–288.
https://doi.org/10.1016/j.virol.2010.08.
015
13. Aceytuno RD, Piett CG, Havali-Shahriari Z,
Edwards RA, Rey M, Ye R, Javed F, Fang S,
Mani R, Weinfeld M, Hammel M, Tainer JA,
Schriemer DC, Lees-Miller SP, Glover JNM
(2017) Structural and functional characterization of the PNKP-XRCC4-LigIV DNA repair
complex.
Nucleic
Acids
Res
45
(10):6238–6251. https://doi.org/10.1093/
nar/gkx275
14. Hsiao HH, Nath A, Lin CY, Folta-Stogniew
EJ, Rhoades E, Braddock DT (2010) Quantitative characterization of the interactions
among c-myc transcriptional regulators
FUSE, FBP, and FIR. Biochemistry 49
(22):4620–4634. https://doi.org/10.1021/
bi9021445
15. French RL, Gupta N, Copeland PR, Simonovic
M (2014) Structural asymmetry of the terminal
catalytic complex in selenocysteine synthesis. J
Biol Chem 289(42):28783–28794. https://
doi.org/10.1074/jbc.M114.597955
16. Aik WS, Lin MH, Tan D, Tripathy A, Marzluff
WF, Dominski Z, Chou CY, Tong L (2017)
The N-terminal domains of FLASH and
Lsm11 form a 2:1 heterotrimer for histone
pre-mRNA 3
0 -end processing. PLoS One 12
(10):e0186034. https://doi.org/10.1371/
journal.pone.0186034
17. Wen J, Arakawa T, Wypych J, Langley KE,
Schwartz MG, Philo JS (1997) Chromatographic determination of extinction coefficients of non-glycosylated proteins using
refractive index (RI) and UV absorbance
(UV) detectors: applications for studying protein interactions by size exclusion chromatography with light-scattering, UV, and RI
detectors. In: Marshak DR (ed) Techniques in
protein chemistry, vol 8. Academic, New York,
pp
113–119.
https://doi.org/10.1016/
S1080-8914(97)80014-2
18. Stevens FJ (1989) Analysis of protein-protein
interaction by simulation of small-zone size
exclusion chromatography. Stochastic formulation of kinetic rate contributions to observed
high-performance liquid chromatography elution
characteristics.
Biophys
J
55
(6):1155–1167. https://doi.org/10.1016/
s0006-3495(89)82912-1
19. Giddings J (1993) Field-flow fractionation:
analysis of macromolecular, colloidal, and particulate
materials.
Science
260
(5113):1456–1465.
https://doi.org/10.
1126/science.8502990
20. Ashby J, Schachermeyer S, Duan Y, Jimenez
LA, Zhong W (2014) Probing and quantifying
DNA–protein interactions with asymmetrical
flow field-flow fractionation. J Chromatogr A
1358:217–224. https://doi.org/10.1016/j.
chroma.2014.07.002
21. Kendrick BS, Kerwin BA, Chang BS, Philo JS
(2001) Online size-exclusion high-performance liquid chromatography light scattering
and differential refractometry methods to
determine degree of polymer conjugation to
proteins and protein-protein or protein-ligand
association states. Anal Biochem 299
(2):136–146. https://doi.org/10.1006/abio.
2001.5411
22. Zhao H, Brown PH, Schuck P (2011) On the
distribution of protein refractive index increments. Biophys J 100(9):2309–2317. https://
doi.org/10.1016/j.bpj.2011.03.004
23. Nishino T, Rago F, Hori T, Tomii K, Cheeseman IM, Fukagawa T (2013) CENP-T provides a structural platform for outer
kinetochore
assembly.
EMBO
J
32
(3):424–436.
https://doi.org/10.1038/
emboj.2012.348
SEC Coupled with Light Scattering
395