7. Huang X, Aulabaugh A (2016) Application of
fluorescence polarization in HTS assays. Methods Mol Biol 1439:115–130
8. Lea W, Simeonov A (2011) Fluorescence polarization assays in small molecule screening.
Expert Opin Drug Discovery 6:17–32
9. Gradinaru C, Marushchak D, Samim M et al
(2010) Fluorescence anisotropy: from single
molecules to live cells. Analyst 135:452–459
10. Pope A, Haupts U, Moore K (1999) Homogeneous fluorescence readouts for miniaturized
high-throughput screening: theory and practice. Drug Discov Today 4:350–362
11. Johnson I, Spence M (2010) The molecular
probes handbook: a guide to fluorescent
probes and labeling technologies 11th edition.
Life Technologies, Carlsbad, California
12. van de Hulst H (1981) Light scattering by
small particles. Dover Publications Inc.,
New York
13. Buchli R, VanGundy RS, Hickman-Miller HD
et al (2005) Development and validation of a
fluorescence polarization-based competitive
peptide-binding assay for HLA-A*0201–a
new tool for epitope discovery. Biochemistry
44:12491–12507
14. Hall M, Yasgar A, Peryea T et al (2016) Fluorescence polarization assays in highthroughput screening and drug discovery: a
review. Methods Appl Fluoresc 4(2):022001
15. Dandliker W, Hsu M, Levin J et al (1981)
Equilibrium and kinetic inhibition assays
based upon fluorescence polarization. Methods
Enzymol 74. Pt C:3–28
16. Cheng Y, Prusoff W (1973) Relationship
between the inhibition constant (K1) and the
concentration of inhibitor which causes 50 per
cent inhibition (I50) of an enzymatic reaction.
Biochem Pharmacol 22(23):3099–3108
17. Munson P, Rodbard D (1988) An exact correction to the "Cheng-Prusoff" correction. J
Recept Res 8(1–4):533–546
18. Wang Z (1995) An exact mathematical expression for describing competitive binding of two
different ligands to a protein molecule. FEBS
Lett 360(2):111–114
19. Turconi S, Shea K, Ashman S et al (2001)
Real experiences of uHTS: a prototypic
1536-well fluorescence anisotropy-based
uHTS screen and application of well-level
quality control procedures. J Biomol Screen
26(5):275–290
20. Good N, Winget GD, Winter W et al (1966)
Hydrogen ion buffers for biological research.
Biochemistry 5:467–477
21. Simeonov A, Jadhav A, Thomas CJ et al (2008)
Fluorescence spectroscopic profiling of compound libraries. J Med Chem 51:2363–2371
22. Cer RZ, Mudunuri U, Stephens R, Lebeda
FJ (2009) IC 50 -to-Ki: a web-based tool for
converting IC 50 to Ki values for inhibitors of
enzyme activity and ligand binding. Nucleic
Acids
Res
37(Web
Server
issue):
W441–W445
246
Geoffrey A. Holdgate and Paul E. Hemsley
fluorescence polarization in HTS assays. Methods Mol Biol 1439:115–130
8. Lea W, Simeonov A (2011) Fluorescence polarization assays in small molecule screening.
Expert Opin Drug Discovery 6:17–32
9. Gradinaru C, Marushchak D, Samim M et al
(2010) Fluorescence anisotropy: from single
molecules to live cells. Analyst 135:452–459
10. Pope A, Haupts U, Moore K (1999) Homogeneous fluorescence readouts for miniaturized
high-throughput screening: theory and practice. Drug Discov Today 4:350–362
11. Johnson I, Spence M (2010) The molecular
probes handbook: a guide to fluorescent
probes and labeling technologies 11th edition.
Life Technologies, Carlsbad, California
12. van de Hulst H (1981) Light scattering by
small particles. Dover Publications Inc.,
New York
13. Buchli R, VanGundy RS, Hickman-Miller HD
et al (2005) Development and validation of a
fluorescence polarization-based competitive
peptide-binding assay for HLA-A*0201–a
new tool for epitope discovery. Biochemistry
44:12491–12507
14. Hall M, Yasgar A, Peryea T et al (2016) Fluorescence polarization assays in highthroughput screening and drug discovery: a
review. Methods Appl Fluoresc 4(2):022001
15. Dandliker W, Hsu M, Levin J et al (1981)
Equilibrium and kinetic inhibition assays
based upon fluorescence polarization. Methods
Enzymol 74. Pt C:3–28
16. Cheng Y, Prusoff W (1973) Relationship
between the inhibition constant (K1) and the
concentration of inhibitor which causes 50 per
cent inhibition (I50) of an enzymatic reaction.
Biochem Pharmacol 22(23):3099–3108
17. Munson P, Rodbard D (1988) An exact correction to the "Cheng-Prusoff" correction. J
Recept Res 8(1–4):533–546
18. Wang Z (1995) An exact mathematical expression for describing competitive binding of two
different ligands to a protein molecule. FEBS
Lett 360(2):111–114
19. Turconi S, Shea K, Ashman S et al (2001)
Real experiences of uHTS: a prototypic
1536-well fluorescence anisotropy-based
uHTS screen and application of well-level
quality control procedures. J Biomol Screen
26(5):275–290
20. Good N, Winget GD, Winter W et al (1966)
Hydrogen ion buffers for biological research.
Biochemistry 5:467–477
21. Simeonov A, Jadhav A, Thomas CJ et al (2008)
Fluorescence spectroscopic profiling of compound libraries. J Med Chem 51:2363–2371
22. Cer RZ, Mudunuri U, Stephens R, Lebeda
FJ (2009) IC 50 -to-Ki: a web-based tool for
converting IC 50 to Ki values for inhibitors of
enzyme activity and ligand binding. Nucleic
Acids
Res
37(Web
Server
issue):
W441–W445
246
Geoffrey A. Holdgate and Paul E. Hemsley