13. Vega S, Abian O, Velazquez-Campoy A (2016)
On the link between conformational changes,
ligand binding and heat capacity. Biochim Biophys Acta 1860(5):868–878. https://doi.org/
10.1016/j.bbagen.2015.10.010
14. Burnouf D, Ennifar E, Guedich S, Puffer B,
Hoffmann G, Bec G, Disdier F, Baltzinger M,
Dumas P (2012) kinITC: a new method for
obtaining joint thermodynamic and kinetic
data by isothermal titration calorimetry. J Am
Chem Soc 134(1):559–565. https://doi.org/
10.1021/ja209057d
15. Vander Meulen KA, Horowitz S, Trievel RC,
Butcher SE (2016) Measuring the kinetics of
molecular association by isothermal titration
calorimetry. Methods Enzymol 567:181–213.
https://doi.org/10.1016/bs.mie.2015.08.
012
16. Demarse NA, Killian MC, Hansen LD, Quinn
CF (2013) Determining enzyme kinetics via
isothermal titration calorimetry. Methods Mol
Biol 978:21–30. https://doi.org/10.1007/
978-1-62703-293-3_2
17. Transtrum MK, Hansen LD, Quinn C (2015)
Enzyme kinetics determined by singleinjection isothermal titration calorimetry.
Methods 76:194–200. https://doi.org/10.
1016/j.ymeth.2014.12.003
18. Keller S, Vargas C, Zhao H, Piszczek G, Brautigam CA, Schuck P (2012) High-precision
isothermal
titration
calorimetry
with
automated peak-shape analysis. Anal Chem 84
(11):5066–5073. https://doi.org/10.1021/
ac3007522
19. Mizoue LS, Tellinghuisen J (2004) The role of
backlash in the “first injection anomaly” in isothermal titration calorimetry. Anal Biochem
326(1):125–127. https://doi.org/10.1016/j.
ab.2003.10.048
20. Tellinghuisen J (2012) Designing isothermal
titration calorimetry experiments for the study
of 1:1 binding: problems with the “standard
protocol”. Anal Biochem 424(2):211–220.
https://doi.org/10.1016/j.ab.2011.12.035
21. Markova N, Hallen D (2004) The development of a continuous isothermal titration calorimetric method for equilibrium studies. Anal
Biochem 331(1):77–88. https://doi.org/10.
1016/j.ab.2004.03.022
22. Cooper A, Johnson CM (1994) Introduction
to microcalorimetry and biomolecular energetics. Methods Mol Biol 22:109–124
23. Gill SC, von Hippel PH (1989) Calculation of
protein extinction coefficients from amino acid
sequence data. Anal Biochem 182(2):319–326
24. Pace CN, Vajdos F, Fee L, Grimsley G, Gray T
(1995) How to measure and predict the molar
absorption coefficient of a protein. Protein Sci
4(11):2411–2423. https://doi.org/10.1002/
pro.5560041120
Isothermal Titration Calorimetry
159
On the link between conformational changes,
ligand binding and heat capacity. Biochim Biophys Acta 1860(5):868–878. https://doi.org/
10.1016/j.bbagen.2015.10.010
14. Burnouf D, Ennifar E, Guedich S, Puffer B,
Hoffmann G, Bec G, Disdier F, Baltzinger M,
Dumas P (2012) kinITC: a new method for
obtaining joint thermodynamic and kinetic
data by isothermal titration calorimetry. J Am
Chem Soc 134(1):559–565. https://doi.org/
10.1021/ja209057d
15. Vander Meulen KA, Horowitz S, Trievel RC,
Butcher SE (2016) Measuring the kinetics of
molecular association by isothermal titration
calorimetry. Methods Enzymol 567:181–213.
https://doi.org/10.1016/bs.mie.2015.08.
012
16. Demarse NA, Killian MC, Hansen LD, Quinn
CF (2013) Determining enzyme kinetics via
isothermal titration calorimetry. Methods Mol
Biol 978:21–30. https://doi.org/10.1007/
978-1-62703-293-3_2
17. Transtrum MK, Hansen LD, Quinn C (2015)
Enzyme kinetics determined by singleinjection isothermal titration calorimetry.
Methods 76:194–200. https://doi.org/10.
1016/j.ymeth.2014.12.003
18. Keller S, Vargas C, Zhao H, Piszczek G, Brautigam CA, Schuck P (2012) High-precision
isothermal
titration
calorimetry
with
automated peak-shape analysis. Anal Chem 84
(11):5066–5073. https://doi.org/10.1021/
ac3007522
19. Mizoue LS, Tellinghuisen J (2004) The role of
backlash in the “first injection anomaly” in isothermal titration calorimetry. Anal Biochem
326(1):125–127. https://doi.org/10.1016/j.
ab.2003.10.048
20. Tellinghuisen J (2012) Designing isothermal
titration calorimetry experiments for the study
of 1:1 binding: problems with the “standard
protocol”. Anal Biochem 424(2):211–220.
https://doi.org/10.1016/j.ab.2011.12.035
21. Markova N, Hallen D (2004) The development of a continuous isothermal titration calorimetric method for equilibrium studies. Anal
Biochem 331(1):77–88. https://doi.org/10.
1016/j.ab.2004.03.022
22. Cooper A, Johnson CM (1994) Introduction
to microcalorimetry and biomolecular energetics. Methods Mol Biol 22:109–124
23. Gill SC, von Hippel PH (1989) Calculation of
protein extinction coefficients from amino acid
sequence data. Anal Biochem 182(2):319–326
24. Pace CN, Vajdos F, Fee L, Grimsley G, Gray T
(1995) How to measure and predict the molar
absorption coefficient of a protein. Protein Sci
4(11):2411–2423. https://doi.org/10.1002/
pro.5560041120
Isothermal Titration Calorimetry
159