135
coding RNA having homologous sequences to the repeat sequences (Jinek et al.
2012). The tract-RNA is transcribed separately and then anneals to the precrRNA and results in formation of RNA binate.
• Severing of RNA binates (crRNA paired with tract-RNA) by RNAase III: In
type II CRISPR system, RNase III cuts the dsRNA into small segments of mature
crRNA possessing overhangs or flanks of repeats. Finally a guide RNA of 20
nucleotides is produced by cleavage with nucleases (Deltcheva et al. 2011;
Marraffini and Sontheimer 2010; Terns and Terns 2011; Chylinski et al. 2011;
Karvelis et al. 2013; Carte et al. 2008).
6.3.3 Formation of Multiplex
• RNA binates form a multiplex with Cas9 protein
6.3.4 Recognition of Foreign DNA with the Help
of Protospacer Adjacent Motif
• CrRNA nucleic acid complex cleavage by Cas9: The mature crRNA forms a multiplex with Cas9 protein. When a phage infects a bacterium, the multiplex of crRNA
fragments and Cas9 cleaves the phage DNA. Cas9 recognizes only those sequences
Fig. 6.1 (continued)
6 Role of Gene Editing Tool CRISPR-Cas in the Management of Antimicrobial…
coding RNA having homologous sequences to the repeat sequences (Jinek et al.
2012). The tract-RNA is transcribed separately and then anneals to the precrRNA and results in formation of RNA binate.
• Severing of RNA binates (crRNA paired with tract-RNA) by RNAase III: In
type II CRISPR system, RNase III cuts the dsRNA into small segments of mature
crRNA possessing overhangs or flanks of repeats. Finally a guide RNA of 20
nucleotides is produced by cleavage with nucleases (Deltcheva et al. 2011;
Marraffini and Sontheimer 2010; Terns and Terns 2011; Chylinski et al. 2011;
Karvelis et al. 2013; Carte et al. 2008).
6.3.3 Formation of Multiplex
• RNA binates form a multiplex with Cas9 protein
6.3.4 Recognition of Foreign DNA with the Help
of Protospacer Adjacent Motif
• CrRNA nucleic acid complex cleavage by Cas9: The mature crRNA forms a multiplex with Cas9 protein. When a phage infects a bacterium, the multiplex of crRNA
fragments and Cas9 cleaves the phage DNA. Cas9 recognizes only those sequences
Fig. 6.1 (continued)
6 Role of Gene Editing Tool CRISPR-Cas in the Management of Antimicrobial…