Mr (kDa)
200
116
97
66
55
36
31
21
1
2
3
C
t l B e a d s
B i o t - T A N
C
1
B i o t - T A N
C
1
Δ P D
Z
B M
A)
-Log10 (p-value)
log2 (TANC1 vs TANC1ΔPDZ)
SCRIB
DLG1
SNX27
MAGI3
MPP2
DTNA
CPVL
UTRN
MPDZ
GORASP2
MPP7
MPP6
MPP5
TTC8
LNX2
SNTB1
SNTB2
MAGI1
LIN7A
SNTA1
DNTB
LIN7C
TAX1BP3
RAPGEF6
DLG3
ERBB2IP
SYNJ2BP
PTPN13
CCDC47
SEC23A
UBR3
HSPA1B
HSPA8
PPP1CC CTNNA
DMD
CASK
NUP35
PPP1CA
INADL
B)
Fig. 4 Identification of TANC1 PDZBM partners by peptide pulldown. (a) SDS-PAGE separation of protein
complexes associated to TANC1 peptides. Purified proteins obtained from pulldown with control beads (left),
beads coupled to TANC1 peptide (middle) or to TANC1 lacking the last three amino-acids (right) were
26
Avais M. Daulat et al.
200
116
97
66
55
36
31
21
1
2
3
C
t l B e a d s
B i o t - T A N
C
1
B i o t - T A N
C
1
Δ P D
Z
B M
A)
-Log10 (p-value)
log2 (TANC1 vs TANC1ΔPDZ)
SCRIB
DLG1
SNX27
MAGI3
MPP2
DTNA
CPVL
UTRN
MPDZ
GORASP2
MPP7
MPP6
MPP5
TTC8
LNX2
SNTB1
SNTB2
MAGI1
LIN7A
SNTA1
DNTB
LIN7C
TAX1BP3
RAPGEF6
DLG3
ERBB2IP
SYNJ2BP
PTPN13
CCDC47
SEC23A
UBR3
HSPA1B
HSPA8
PPP1CC CTNNA
DMD
CASK
NUP35
PPP1CA
INADL
B)
Fig. 4 Identification of TANC1 PDZBM partners by peptide pulldown. (a) SDS-PAGE separation of protein
complexes associated to TANC1 peptides. Purified proteins obtained from pulldown with control beads (left),
beads coupled to TANC1 peptide (middle) or to TANC1 lacking the last three amino-acids (right) were
26
Avais M. Daulat et al.